The effect of polymer adsorption on the dispersion stability of particles is an important subject applicable to various manufacturing processes. In this study, small-angle scattering was used to examine the relationship between interparticle potential and polymer adsorption in dispersions of nanoparticles with an 81 Å radius containing two types of polymers. Small-angle X-ray scattering (SAXS) measurements in a silica/polyacrylamide (PAAm) system showed an increase in interparticle attractive interactions as PAAm concentration was increased. In a silica/poly(ethylene oxide) (PEO) system, the correlation between PEO concentration and interparticle potential strength became negligible at higher concentrations. Hence, the contrast variation small-angle neutron scattering (CV-SANS) method was employed to evaluate the interparticle potential and polymer adsorption simultaneously. CV-SANS revealed that PAAm was adsorbed to silica particles with a polymer shell layer thickness of 186 Å. The attractive potential observed in the absorbed layer region can be attributed to bridging PAAm molecules between the silica particles. By contrast, CV-SANS of the silica/PEO system indicated a low-polymer-concentration layer with a thickness of 34 Å around silica particles, indicating weak adsorption of PEO molecules. Negligible interaction between PEO and silica particles was assumed to be the origin of the depletion stabilization from excess polymer addition. Thus, quantitative analyses conducted using SAXS and CV-SANS measurements for the first time clearly demonstrated a difference in the adsorption structure of the polymer, which induces changes in the interaction potential between nanoparticles.