在用于等电聚焦的混合床固定化电解质凝胶中使用多缓冲剂作为载体两性电解质。

Use of polybuffer as carrier ampholytes in mixed-bed Immobiline gels for isoelectric focusing.

作者信息

Rabilloud T, Barzaghi B, Righetti P G

机构信息

Ecole Normale Supérieure, Biology Department, Paris, France.

出版信息

J Biochem Biophys Methods. 1988 Jun-Jul;16(2-3):237-41. doi: 10.1016/0165-022x(88)90034-6.

DOI:10.1016/0165-022x(88)90034-6

PMID:3411086

Abstract

In mixed-bed, carrier ampholyte-Immobiline gels, a primary, insolubilized pH gradient is admixed with a secondary, soluble pH gradient generated by amphoteric buffers. The latter are the standard carrier ampholytes (e.g. Ampholine, Pharmalyte, Biolyte, Servalyte), used in conventional isoelectric focusing, admixed to Immobiline gels in levels of approximately 0.5-1%. It is here shown that polybuffers 96 (covering the pH 6-9 range) and 74 (covering the pH 4-7 interval) used as eluents in chromatofocusing, can effectively substitute the standard carrier ampholytes with considerable savings (they are 1/16th as expensive as the latter chemicals).

摘要

在混合床载体两性电解质-固定化凝胶中，一种初级的不溶性pH梯度与由两性缓冲剂产生的次级可溶性pH梯度混合。后者是常规等电聚焦中使用的标准载体两性电解质（如两性电解质、Pharmalyte、Biolyte、Servalyte），以约0.5-1%的水平与固定化凝胶混合。本文表明，在色谱聚焦中用作洗脱剂的多缓冲剂96（覆盖pH 6-9范围）和74（覆盖pH 4-7区间）可以有效替代标准载体两性电解质，节省大量成本（它们的价格仅为后者化学品的1/16）。