1Department of Parasitology and Zoology, University of Veterinary Medicine, Budapest, Hungary.
2Plant Protection Institute, Centre for Agricultural Research, ELKH, Budapest, Hungary.
Acta Vet Hung. 2021 Jun 9;69(2):157-160. doi: 10.1556/004.2021.00017.
In this study, faecal samples of four American Staffordshire terrier dogs (used for illegal fighting) were analysed by DNA extraction, molecular-phylogenetic and parasitological methods, in order to examine the occurrence of protozoan, apicomplexan parasites. In one sample, the DNA of Sarcocystis morae was shown to be present. This species was identified based on 100% identity with already reported sequences of S. morae from cervids in Lithuania and Spain. The result was also confirmed by phylogenetic analysis. The sporocysts of the canine S. morae isolate measured 14.95 × 9.75 μm on average. This is the first molecular evidence in support of the final host role of domestic dogs in the life cycle of S. morae. The most likely source of the infection was raw meat given to the examined dog to increase its physical achievement. In conclusion, under similar circumstances dogs may participate in the life cycle of S. morae in a 'natural way', shedding sporocysts/oocysts when used for hunting or taken to walks in forested areas.
在这项研究中,通过 DNA 提取、分子系统发育和寄生虫学方法分析了四只美国斯塔福德郡梗犬(用于非法战斗)的粪便样本,以检查原生动物、顶复门寄生虫的发生情况。在一个样本中,检测到莫氏肉孢子虫的 DNA。根据与立陶宛和西班牙鹿源莫氏肉孢子虫已报道序列的 100%同一性,对该物种进行了鉴定。这一结果也通过系统发育分析得到了证实。犬源莫氏肉孢子虫分离株的孢子囊平均大小为 14.95×9.75μm。这是支持家犬在莫氏肉孢子虫生活史中终末宿主作用的第一个分子证据。感染的最可能来源是给予检查犬的生肉,以提高其身体素质。总之,在类似情况下,当狗用于狩猎或在森林地区散步时,它们可能会以“自然方式”参与莫氏肉孢子虫的生活史,排出孢子囊/卵囊。