Berra Yanina, Moré Gastón, Helman Elisa, Argibay Hernan D, Orozco M Marcela
Cátedra de Salud Pública, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, Chorroarin 280, (C1217DSM), Ciudad Autónoma de Buenos Aires, Argentina.
Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Godoy Cruz 2290, (C1425FQB), Ciudad Autónoma de Buenos Aires, Argentina.
Int J Parasitol Parasites Wildl. 2023 Jan 2;20:39-45. doi: 10.1016/j.ijppaw.2022.12.007. eCollection 2023 Apr.
The marsh deer () is the largest South American native deer species and is listed as "Vulnerable" by IUCN due to the population reduction. As part of a conservation and disease surveillance program, muscle samples from 14 marsh deer found dead in 2016 and 2017 in northeast Argentina were obtained at necropsy. Samples from each animal were processed as pooled muscles (heart, diaphragm, tongue and hindlimb) by homogenization and direct microscopical observation to detect intracellular spp. cysts. Sarcocysts were observed in six samples, and several cysts recovered from two samples were processed by transmission electron microscopy. The cysts were thin-walled and showed a cyst-wall ultrastructure with ribbon-like protrusions similar to other species using cervids as intermediate host and canids as definitive hosts. Genomic DNA from individual sarcocysts from three marsh deer were successfully amplified by PCR of and gene fragments and further sequenced. Sequence comparison revealed a 99.3-100% identity among them and only 93.7-96.6% and 88.8-89.7% identity at and markers, respectively, with other spp. Despite morphological similarities, the high sequence divergence at and fragments allowed the assumption that sp. from marsh deer is a different species from others using cervids as intermediate hosts. Therefore, we propose the name n. sp. for the species infecting marsh deer.
草原鹿（）是南美洲最大的本土鹿种，由于种群数量减少，被国际自然保护联盟列为“易危”物种。作为保护和疾病监测计划的一部分，2016年和2017年在阿根廷东北部发现的14只死亡草原鹿的肌肉样本在尸检时被采集。将每只动物的样本作为混合肌肉（心脏、膈肌、舌头和后肢）进行处理，通过匀浆和直接显微镜观察来检测细胞内的 spp. 囊肿。在6个样本中观察到了肉孢子虫囊肿，从2个样本中回收的几个囊肿通过透射电子显微镜进行处理。这些囊肿壁薄，显示出一种囊壁超微结构，带有带状突起，类似于其他以鹿科动物为中间宿主、犬科动物为终末宿主的物种。通过对 和 基因片段的PCR成功扩增了来自三只草原鹿个体肉孢子虫的基因组DNA，并进一步进行了测序。序列比较显示它们之间的同一性为99.3 - 100%，而在 和 标记处与其他 spp. 的同一性分别仅为93.7 - 96.6%和88.8 - 89.7%。尽管形态相似，但 和 片段的高序列差异表明，草原鹿的 sp. 是一种与其他以鹿科动物为中间宿主的物种不同的物种。因此，我们提议将感染草原鹿的物种命名为 n. sp.。
