利用粳稻和籼稻品种间 F1 代植物的 RNA 测序进行低温耐受性相关转录本的选择。

Selection of transcripts related to low-temperature tolerance using RNA sequencing from F plants between japonica and indica rice (Oryza sativa L.) cultivars.

机构信息

Institute of Crop Science, National Agriculture and Food Research Organization, Tsukuba, Ibaraki, Japan; and Corresponding author. Email:

Faculty of Agriculture, Takasaki University of Health and Welfare, Takasaki, Gunma, Japan.

出版信息

Funct Plant Biol. 2021 Sep;48(10):984-993. doi: 10.1071/FP21088.

DOI:10.1071/FP21088

PMID:34112311

Abstract

At low temperatures (18°C), seedlings of an indica rice (Oryza sativa L.) cultivar Kasalath showed symptoms of chlorosis, although the leaves of a japonica cultivar Arroz da Terra remained green. In this study, transcripts related to the chlorophyll content of rice seedlings grown at 18°C were investigated using RNA-sequencing (RNA-Seq) data for F2 crosses between cultivars Arroz da Terra and Kasalath, as well as their parental cultivars. Differential expression analysis revealed that gene ontology terms related to 'photosynthesis' were significantly enriched in lowly expressed genes at 18°C than at 25°C in Kasalath. However, the gene ontology terms related to 'response to stress' were significantly enriched in highly expressed genes at 18°C than at 25°C in Kasalath. When the F2 plants were grown at 18°C, their chlorophyll contents varied. Transcripts with expression levels related to chlorophyll content were statistically selected using RNA-Seq data from 21 F2 plants. In regression models, frequently selected genes included four photosynthetic and two stress-responsive genes. The expression values of four photosynthetic and two stress-responsive genes in high-frequency selected genes were significantly correlated with chlorophyll content not only in plants analysed using RNA-Seq but also in 95 F2 plants.

摘要

在低温（18°C）下，籼稻品种 Kasalath 的幼苗表现出黄化症状，而粳稻品种 Arroz da Terra 的叶片则保持绿色。在这项研究中，使用 RNA 测序（RNA-Seq）数据，对籼稻品种 Arroz da Terra 和 Kasalath 及其亲本品种之间的 F2 杂交后代在 18°C 下生长的幼苗的与叶绿素含量相关的转录本进行了研究。差异表达分析表明，在 Kasalath 中，与“光合作用”相关的基因本体术语在 18°C 下的低表达基因中显著富集，而在 25°C 下则不富集。然而，在 Kasalath 中，与“应激反应”相关的基因本体术语在 18°C 下的高表达基因中显著富集，而在 25°C 下则不富集。当 F2 植株在 18°C 下生长时，它们的叶绿素含量发生了变化。使用来自 21 株 F2 植株的 RNA-Seq 数据，对与叶绿素含量相关的表达水平的转录本进行了统计选择。在回归模型中，经常选择的基因包括四个光合作用和两个应激反应基因。在高频率选择的基因中，四个光合作用和两个应激反应基因的表达值不仅与使用 RNA-Seq 分析的植物的叶绿素含量显著相关，而且与 95 株 F2 植物的叶绿素含量也显著相关。