Nguyen Dinh Sy, Sai Than Zaw Tun, Nawaz Ghazala, Lee Kwanuk, Kang Hunseung
Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea; Institute of Environment and Biotechnology, Taynguyen University, 567 Le Duan Street, Buon Ma Thuot City, Daklak Province, Viet Nam.
Department of Plant Biotechnology, College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.
J Plant Physiol. 2016 Aug 20;201:85-94. doi: 10.1016/j.jplph.2016.07.004. Epub 2016 Jul 11.
Despite the increasing understanding of the regulation of chloroplast gene expression in plants, the importance of intron splicing and processing of chloroplast RNA transcripts under stress conditions is largely unknown. Here, to understand how abiotic stresses affect the intron splicing and expression patterns of chloroplast genes in dicots and monocots, we carried out a comprehensive analysis of the intron splicing and expression patterns of chloroplast genes in the coffee plant (Coffea arabica) as a dicot and rice (Oryza sativa) as a monocot under abiotic stresses, including drought, cold, or combined drought and heat stresses. The photosynthetic activity of both coffee plants and rice seedlings was significantly reduced under all stress conditions tested. Analysis of the transcript levels of chloroplast genes revealed that the splicing of tRNAs and mRNAs in coffee plants and rice seedlings were significantly affected by abiotic stresses. Notably, abiotic stresses affected differently the splicing of chloroplast tRNAs and mRNAs in coffee plants and rice seedlings. The transcript levels of most chloroplast genes were markedly downregulated in both coffee plants and rice seedlings upon stress treatment. Taken together, these results suggest that coffee and rice plants respond to abiotic stresses via regulating the intron splicing and expression of different sets of chloroplast genes.
尽管人们对植物叶绿体基因表达调控的理解日益加深，但在胁迫条件下叶绿体RNA转录本的内含子剪接和加工的重要性仍 largely未知。在此，为了解非生物胁迫如何影响双子叶植物和单子叶植物中叶绿体基因的内含子剪接和表达模式，我们对咖啡植株（双子叶植物阿拉伯咖啡）和水稻（单子叶植物水稻）在包括干旱、寒冷或干旱与高温联合胁迫等非生物胁迫下叶绿体基因的内含子剪接和表达模式进行了全面分析。在所有测试的胁迫条件下，咖啡植株和水稻幼苗的光合活性均显著降低。对叶绿体基因转录水平的分析表明，咖啡植株和水稻幼苗中tRNA和mRNA的剪接受非生物胁迫的显著影响。值得注意的是，非生物胁迫对咖啡植株和水稻幼苗中叶绿体tRNA和mRNA的剪接影响不同。胁迫处理后，咖啡植株和水稻幼苗中大多数叶绿体基因的转录水平均显著下调。综上所述，这些结果表明咖啡和水稻植株通过调控不同组叶绿体基因的内含子剪接和表达来响应非生物胁迫。
