State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China.
Food Res Int. 2021 Jul;145:110393. doi: 10.1016/j.foodres.2021.110393. Epub 2021 May 14.
Our previous study has demonstrated that sulfated yam polysaccharide (SCYP) had a stronger immunomodulatory activity than yam polysaccharide (CYP). In order to investigate how the sulfated modification influence the immunological activity of CYP, this research was mainly focused on the study of gut microbiotal. The results showed that SCYP treatment could increase the digestive enzyme activities of colon contents and restore the production of short-chain fatty acids (SCFAs) in mice that were decreased by cyclophosphamide (Cy) treatment. Furthermore, SCYP treatment could modulate the structure of the gut microbiota, which was mainly manifest in the result of an increased abundances of Lactobacillus, Bacteroidetes and Akkermansia, and the decrease of the proportion of Proteobacteria and Verrucomicrobia in the microbial community. Diversity and overall structure of microbial community were also improved by SCYP treatment based on alpha-diversity and beta-diversity analysis results. The biomarkers of the gut microbial that were regulated by SCYP have been identified by linear discriminant analysis (LDA) effect size (LEfSe). These findings further indicate that there is great potential for SCYP to be developed into prebiotics or functional foods.
我们之前的研究表明，硫酸山药多糖（SCYP）比山药多糖（CYP）具有更强的免疫调节活性。为了研究硫酸化修饰如何影响 CYP 的免疫活性，本研究主要集中在肠道微生物群的研究上。结果表明，SCYP 处理可增加结肠内容物的消化酶活性，并恢复环磷酰胺（Cy）处理降低的短链脂肪酸（SCFA）的产生。此外，SCYP 处理可调节肠道微生物群的结构，主要表现为乳杆菌、拟杆菌门和阿克曼氏菌的丰度增加，而微生物群落中变形菌门和疣微菌门的比例降低。基于 alpha 多样性和 beta 多样性分析结果，SCYP 处理还改善了微生物群落的多样性和整体结构。通过线性判别分析（LDA）效应大小（LEfSe）鉴定出受 SCYP 调节的肠道微生物生物标志物。这些发现进一步表明，SCYP 具有开发为益生元或功能性食品的巨大潜力。
