Schumacher M, Sulon J, Balthazart J
Laboratoire de Biochimie Générale et Comparée, Université de Liège, Belgium.
J Endocrinol. 1988 Jul;118(1):127-34. doi: 10.1677/joe.0.1180127.
Serum concentrations of testosterone, 5 alpha-dihydrotestosterone, oestradiol and progesterone were measured by radioimmunoassay combined with Celite chromatography in male and female Japanese quail (Coturnix coturnix japonica) during the second half of embryonic life (days 9-17 of incubation) and during the first 5 weeks after hatching. The mean level of each of the four steroids was significantly affected by the age of the birds. An overall effect of sex was detected by analysis of variance only on oestradiol concentrations, with females having higher serum concentrations than males during most of the age range studied. Significant peaks of testosterone and progesterone were also detected around hatching time. These results are consistent with the view that oestradiol is the major hormone implicated in the sexual differentiation of reproductive behaviour in the quail. The relationships between the circulating concentrations of oestradiol during ontogeny and the critical period of differentiation as postulated by currently accepted models is also discussed.
在日本鹌鹑（Coturnix coturnix japonica）胚胎发育后半期（孵化第9 - 17天）及孵化后前5周，通过放射免疫分析法结合硅藻土色谱法测定了雄性和雌性鹌鹑血清中睾酮、5α - 二氢睾酮、雌二醇和孕酮的浓度。这四种类固醇激素的平均水平均受到鹌鹑年龄的显著影响。仅通过方差分析检测到性别对雌二醇浓度有总体影响，在所研究的大部分年龄范围内，雌性血清浓度高于雄性。在孵化期前后还检测到睾酮和孕酮的显著峰值。这些结果与雌二醇是参与鹌鹑生殖行为性别分化的主要激素这一观点一致。本文还讨论了个体发育过程中雌二醇循环浓度与当前公认模型所假设的关键分化期之间的关系。
