Gao Caixia, Qiao Tiankui, Yuan Sujuan, Zhuang Xibing
Jinshan Hospital Center for Tumor Diagnosis & Therapy, Shanghai, 201508, People's Republic of China.
Cancer Manag Res. 2021 Jun 4;13:4497-4507. doi: 10.2147/CMAR.S305452. eCollection 2021.
Many studies have reported that the inflammatory immune response related to signaling activation participates in tumor development and affects the treatment outcome. functions as a tumor suppressor by regulating DNA methylation. protein plays an important role in TGF-β signaling pathway that is involved in tumor growth inhibition and apoptosis. At present, radiotherapy is still an important treatment in lung cancer, which induces immune response and affects the therapeutic outcome. The role of signaling activation and in this process is not clear.
In this study, we investigated the expression of in tumor and in surrounding tissues by immunohistochemical methods and analyzed the relationship on postoperative survival in lung cancer.
We found that the high expression of was the risk factor in postoperative survival of lung cancer with no difference in lifetime. The high expression of in lung cancer with signaling activation was in favor of progression-free survival and overall survival in postoperative radiotherapy. It suggested that played an important role in lung cancer radiotherapy. In order to determine the effect of in lung cancer radiation with signaling activation, we introduced 5-Aza-2'-deoxycytidine (5-Aza-CdR) and exposed lung cancer A459 cells repeatedly. The high expression of especially -B in cells treated with 5-Aza-CdR was observed. We examined that 5-Aza-CdR induced more cell blocking in G2/M phase in combining irradiation.
The result implied that it was feasible to improve radiosensitivity of lung cancer with signaling activation by increasing expression, and 5-Aza-CdR was an option in this process.
许多研究报道，与信号激活相关的炎症免疫反应参与肿瘤发展并影响治疗结果。 通过调节DNA甲基化发挥肿瘤抑制作用。 蛋白在参与肿瘤生长抑制和凋亡的转化生长因子-β（TGF-β）信号通路中起重要作用。目前，放射治疗仍是肺癌的重要治疗方法，其可诱导免疫反应并影响治疗结果。信号激活和 在这一过程中的作用尚不清楚。
在本研究中，我们通过免疫组织化学方法研究了肿瘤中 和周围组织中 的表达，并分析了其与肺癌术后生存的关系。
我们发现， 的高表达是肺癌术后生存的危险因素，生存期无差异。 在有信号激活的肺癌中的高表达有利于术后放疗的无进展生存和总生存。这表明 在肺癌放疗中起重要作用。为了确定 在有信号激活的肺癌放疗中的作用，我们引入5-氮杂-2'-脱氧胞苷（5-Aza-CdR）并反复处理肺癌A459细胞。观察到在5-Aza-CdR处理的细胞中 尤其是 -B的高表达。我们检测到5-Aza-CdR在联合照射时诱导更多细胞阻滞于G2/M期。
结果表明，通过增加 表达来提高有信号激活的肺癌的放射敏感性是可行的，并且5-Aza-CdR是这一过程中的一种选择。
