Longo Elena, Sancey Lucie, Cedola Alessia, Barbier Emmanuel L, Bravin Alberto, Brun Francesco, Bukreeva Inna, Fratini Michela, Massimi Lorenzo, Greving Imke, Le Duc Geraldine, Tillement Olivier, De La Rochefoucauld Ombeline, Zeitoun Philippe
Helmholtz-Zentrum Hereon, Institute of Materials Physics, Geesthacht, Germany.
Laboratoire d'Optique Appliquée UMR7639, ENSTA-CNRS-Ecole Polytechnique IP Paris, Palaiseau, France.
Front Oncol. 2021 May 25;11:554668. doi: 10.3389/fonc.2021.554668. eCollection 2021.
Characterizing nanoparticles (NPs) distribution in multiple and complex metastases is of fundamental relevance for the development of radiological protocols based on NPs administration. In the literature, there have been advances in monitoring NPs in tissues. However, the lack of 3D information is still an issue. X-ray phase-contrast tomography (XPCT) is a 3D label-free, non-invasive and multi-scale approach allowing imaging anatomical details with high spatial and contrast resolutions. Here an XPCT qualitative study on NPs distribution in a mouse brain model of melanoma metastases injected with gadolinium-based NPs for theranostics is presented. For the first time, XPCT images show the NPs uptake at micrometer resolution over the full brain. Our results revealed a heterogeneous distribution of the NPs inside the melanoma metastases, bridging the gap in spatial resolution between magnetic resonance imaging and histology. Our findings demonstrated that XPCT is a reliable technique for NPs detection and can be considered as an emerging method for the study of NPs distribution in organs.
表征纳米颗粒（NPs）在多种复杂转移灶中的分布，对于基于纳米颗粒给药的放射学方案的开发具有根本意义。在文献中，在监测组织中的纳米颗粒方面已有进展。然而，缺乏三维信息仍然是一个问题。X射线相衬断层扫描（XPCT）是一种无标记、非侵入性的多尺度三维方法，能够以高空间分辨率和对比度分辨率对解剖细节进行成像。本文展示了一项关于XPCT的定性研究，该研究针对注射了用于诊疗的钆基纳米颗粒的黑色素瘤转移小鼠脑模型中的纳米颗粒分布情况。首次，XPCT图像以微米分辨率显示了整个大脑中纳米颗粒的摄取情况。我们的结果揭示了纳米颗粒在黑色素瘤转移灶内的异质性分布，弥合了磁共振成像和组织学之间空间分辨率的差距。我们的研究结果表明，XPCT是一种可靠的纳米颗粒检测技术，可被视为研究纳米颗粒在器官中分布的一种新兴方法。
