National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-environmental Pollution Control and Management, Institute of Eco-environmental and Soil Sciences, Guangdong Academy of Sciences, 808 Tianyuan Road, Guangzhou, 510650, Guangdong, China.
School of Environment, Henan Normal University, Xinxiang, Henan, China.
Environ Sci Pollut Res Int. 2021 Nov;28(41):58523-58535. doi: 10.1007/s11356-021-14595-x. Epub 2021 Jun 11.
Soil contamination due to mining activities is a great concern in China. Although the effects of mining pollution resulting in changes of soil characteristics and the microbiome have been documented, studies on the responses of plant root-associated microbial assemblages remain scarce. In this work, we collected bulk soil, rhizosphere soil, and root endosphere samples of Cyperus rotundus L (Cyp) plants from two Pb/Zn mines, of which, one was abandoned (SL) and the other was active (GD), to investigate the bacterial community responses across different site contamination levels and Cyp plant compartments. For comparison, one unpolluted site (SD) was included. Results revealed that soils from the SL and GD sites were seriously contaminated by metal(loid)s, including Pb, Zn, As, and Sb. Bacterial richness and diversity depended on the sampling site and plant compartment. All sample types from the SL site had the lowest bacterial diversities and their bacterial communities also exhibited distinct patterns compared to GD and SD samples. As for the specific sampling site, bacterial communities from the root endosphere exhibited different patterns from those in bulk and rhizosphere soil. Compared to the GD and SD sites, the root endosphere and the rhizosphere soil from the SL site shared core microbes, including Halomonas, Pelagibacterium, and Chelativorans, suggesting that they play key roles in Cyp plant survival in such harsh environments.
采矿活动导致的土壤污染在中国是一个令人关注的问题。尽管已经有研究记录了采矿污染导致土壤特性和微生物组变化的影响，但对于植物根际微生物群落的响应研究仍然很少。在这项工作中，我们从两个 Pb/Zn 矿收集了香蒲（Cyp）植物的原状土壤、根际土壤和根内圈样本，其中一个是废弃的（SL），另一个是活跃的（GD），以研究不同污染水平和 Cyp 植物区系的细菌群落响应。为了比较，还包括一个未受污染的地点（SD）。结果表明，SL 和 GD 地点的土壤受到金属（类）如 Pb、Zn、As 和 Sb 的严重污染。细菌丰富度和多样性取决于采样地点和植物区系。来自 SL 地点的所有样本类型的细菌多样性最低，其细菌群落与 GD 和 SD 样本的模式也明显不同。就特定的采样地点而言，根内圈的细菌群落与原状土壤和根际土壤的模式不同。与 GD 和 SD 地点相比，SL 地点的根内圈和根际土壤共享核心微生物，包括盐单胞菌、海杆菌属和Chelativorans，这表明它们在 Cyp 植物在如此恶劣的环境中的生存中发挥关键作用。
