核糖体蛋白L12和L10以及延伸因子Tu的mRNA依赖性体外合成
mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu.
作者信息
Chu F, Caldwell P, Weissbach H, Brot N
出版信息
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5387-91. doi: 10.1073/pnas.74.12.5387.
Abstract
RNA extracted from growing Escherichia coli can direct the in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu when an E. coli system is used. The synthesized L12 can be bound to L12-depleted ribosomes and the synthesized elongation factor Tu can form complexes with both elongation factor Ts and GDP. Guanosine 5'-diphosphate 3'-diphosphate has no effect on the synthesis of these proteins from an RNA template but inhibits their synthesis when a DNA template is used.
摘要
当使用大肠杆菌系统时，从生长中的大肠杆菌中提取的RNA能够指导核糖体蛋白L12和L10以及延伸因子Tu的体外合成。合成的L12可以与缺乏L12的核糖体结合，合成的延伸因子Tu可以与延伸因子Ts和GDP形成复合物。5'-二磷酸鸟苷3'-二磷酸对从RNA模板合成这些蛋白质没有影响，但当使用DNA模板时会抑制它们的合成。
相似文献
1
mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu.核糖体蛋白L12和L10以及延伸因子Tu的mRNA依赖性体外合成
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5387-91. doi: 10.1073/pnas.74.12.5387.
2
Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro.体外大肠杆菌核糖体蛋白L10合成的自体调控
Proc Natl Acad Sci U S A. 1980 May;77(5):2592-5. doi: 10.1073/pnas.77.5.2592.
3
Studies on stringent control in a cell-free system. Regulation by guanosine-5'-diphosphate-3'-diphosphate of the synthesis of elongation factor Tu.无细胞系统中严紧控制的研究。鸟苷-5'-二磷酸-3'-二磷酸对延伸因子Tu合成的调控
J Biochem. 1979 Aug;86(2):403-11. doi: 10.1093/oxfordjournals.jbchem.a132539.
4
The mRNA-directed synthesis of the alpha0peptide of beta-galactosidase, ribosomal proteins L12 and L10, and elongation factor Tu, using purified translational factors.利用纯化的翻译因子，以信使核糖核酸（mRNA）为模板合成β-半乳糖苷酶的α0肽、核糖体蛋白L12和L10以及延伸因子Tu。
Arch Biochem Biophys. 1978 Apr 30;187(2):457-63. doi: 10.1016/0003-9861(78)90057-7.
5
Guanosinetriphosphatase activity dependent on elongation factor Tu and ribosomal protein L7/L12.依赖延伸因子Tu和核糖体蛋白L7/L12的鸟苷三磷酸酶活性
Proc Natl Acad Sci U S A. 1978 Jul;75(7):3192-5. doi: 10.1073/pnas.75.7.3192.
6
Escherichia coli stringent factor binds to ribosomes at a site different from that of elongation factor Tu or G.大肠杆菌严谨因子与核糖体的结合位点不同于延伸因子Tu或G的结合位点。
Biochemistry. 1975 Oct 7;14(20):4414-20. doi: 10.1021/bi00691a012.
7
A single-headed dimer of Escherichia coli ribosomal protein L7/L12 supports protein synthesis.大肠杆菌核糖体蛋白L7/L12的单头二聚体支持蛋白质合成。
Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4215-8. doi: 10.1073/pnas.95.8.4215.
8
The L7/L12 proteins change their conformation upon interaction of EF-G with ribosomes.当EF-G与核糖体相互作用时，L7/L12蛋白会改变其构象。
FEBS Lett. 1984 Oct 15;176(1):32-6. doi: 10.1016/0014-5793(84)80906-0.
9
Dimer state of protein L7/L12 and EF-G-dependent reactions of ribosomes.蛋白质L7/L12的二聚体状态与核糖体的EF-G依赖性反应。
Eur J Biochem. 1978 Oct;90(2):319-23. doi: 10.1111/j.1432-1033.1978.tb12607.x.
10
Cross-linking of selected residues in the N- and C-terminal domains of Escherichia coli protein L7/L12 to other ribosomal proteins and the effect of elongation factor Tu.大肠杆菌L7/L12蛋白N端和C端结构域中特定残基与其他核糖体蛋白的交联以及延伸因子Tu的作用
J Biol Chem. 1998 Jan 16;273(3):1670-6. doi: 10.1074/jbc.273.3.1670.
引用本文的文献
1
Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro.体外大肠杆菌核糖体蛋白L10合成的自体调控
Proc Natl Acad Sci U S A. 1980 May;77(5):2592-5. doi: 10.1073/pnas.77.5.2592.
2
The search for guanosine tetraphosphate (ppGpp) and other unusual nucleotides in eucaryotes.真核生物中鸟苷四磷酸（ppGpp）及其他异常核苷酸的研究。
Microbiol Rev. 1979 Mar;43(1):27-41. doi: 10.1128/mr.43.1.27-41.1979.
3
In vitro regulation of DNA-dependent synthesis of Escherichia coli ribosomal protein L12.大肠杆菌核糖体蛋白L12的DNA依赖性合成的体外调控
Proc Natl Acad Sci U S A. 1979 Apr;76(4):1716-20. doi: 10.1073/pnas.76.4.1716.
4
Eukaryotic ribosomal proteins stimulate Escherichia coli stringent factor to synthesize guanosine 5'-diphosphate, 3'-diphosphate (ppGpp) and guanosine 5'-triphosphate, 3'-diphosphate (ppGpp).真核生物核糖体蛋白刺激大肠杆菌严谨因子合成鸟苷5'-二磷酸-3'-二磷酸（ppGpp）和鸟苷5'-三磷酸-3'-二磷酸（pppGpp）。
Mol Gen Genet. 1978 Nov 9;166(3):291-7. doi: 10.1007/BF00267621.
本文引用的文献
1
DNA-directed in vitro synthesis of elongation factor Tu.DNA 指导的延伸因子 Tu 的体外合成
Biochem Biophys Res Commun. 1976 Dec 20;73(4):917-27. doi: 10.1016/0006-291x(76)90209-6.
2
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
3
THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.大肠杆菌中指定核糖体蛋白的染色体位点。
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1367-74. doi: 10.1073/pnas.52.6.1367.
4
In vitro synthesis of bacteriophage lysozyme.噬菌体溶菌酶的体外合成
Nature. 1967 Aug 5;215(5101):588-91. doi: 10.1038/215588a0.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Radioactive labeling of proteins in vitro.蛋白质的体外放射性标记
J Biol Chem. 1971 Feb 10;246(3):831-2.
7
DNA-dependent in vitro synthesis of bacteriophage enzymes.噬菌体酶的DNA依赖性体外合成。
Cold Spring Harb Symp Quant Biol. 1969;34:763-6. doi: 10.1101/sqb.1969.034.01.086.
8
Cell-free synthesis of tryptophanase from Escherichia coli. Use of ribonucleic acid isolated from induced cells and a comparison of the product from a system employing ribosomes with that from one employing ribosomes and exogenous ribonucleic acid.大肠杆菌色氨酸酶的无细胞合成。使用从诱导细胞中分离的核糖核酸，并比较采用核糖体的系统与采用核糖体和外源核糖核酸的系统所产生的产物。
Biochem J. 1971 Nov;125(2):643-53. doi: 10.1042/bj1250643.
9
Purification of factor Ts: studies on the formation and stability of nucleotide complexes containing transfer factor Tu.因子 Ts 的纯化：关于含转移因子 Tu 的核苷酸复合物的形成与稳定性的研究
Arch Biochem Biophys. 1971 Dec;147(2):457-66. doi: 10.1016/0003-9861(71)90401-2.
10
In vitro synthesis of Escherichia coli alkaline phosphatase monomers.大肠杆菌碱性磷酸酶单体的体外合成
J Mol Biol. 1971 Jun 14;58(2):469-79. doi: 10.1016/0022-2836(71)90364-0.
Zotero 插件