一种用于对动物、人类和环境样本进行快速病毒筛查的全冠状病毒逆转录聚合酶链反应检测方法。
A pan-coronavirus RT-PCR assay for rapid viral screening of animal, human, and environmental specimens.
作者信息
Wang Xinye, Xiu Leshan, Binder Raquel A, Toh Teck-Hock, Lee Jeffrey Soon-Yit, Ting Jakie, Than Son T, Qi Wenhao, Coleman Kristen K, Perera David, Ma Maijuan, Gray Gregory C
机构信息
Global Health Research Center, Duke Kunshan University, Kunshan, China.
School of Medical Sciences, Faculty of Medicine, University of New South Wales, Sydney, NSW, Australia.
出版信息
One Health. 2021 Dec;13:100274. doi: 10.1016/j.onehlt.2021.100274. Epub 2021 Jun 6.
Abstract
We examined a collection of 386 animal, 451 human, and 109 archived bioaerosol samples with a new pan-species coronavirus molecular assay. Thirty-eight (4.02%) of 946 specimens yielded evidence of human or animal coronaviruses. Our findings demonstrate the utility of employing the pan-CoV RT-PCR assay in detecting varied coronavirus among human, animal, and environmental specimens. This RT-PCR assay might be employed as a screening diagnostic for early detection of coronaviruses incursions or prepandemic coronavirus emergence in animal or human populations.
摘要
我们使用一种新的全物种冠状病毒分子检测方法，检测了386份动物样本、451份人类样本和109份存档生物气溶胶样本。在946份标本中，有38份（4.02%）检测出人类或动物冠状病毒阳性。我们的研究结果证明了使用全冠状病毒逆转录聚合酶链反应（RT-PCR）检测方法在检测人类、动物和环境样本中多种冠状病毒的实用性。这种RT-PCR检测方法可作为一种筛查诊断方法，用于早期检测动物或人类群体中冠状病毒的入侵或大流行前冠状病毒的出现。
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