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(硅藻门,圆筛藻科)的完整线粒体基因组及系统发育分析

The complete mitochondrial genome and phylogenetic analysis of (Coscinodiscophyceae, Bacillariophyta).

作者信息

Huang Hailong, Wang Yichao, Song Huiyin, Wang Jing, Chen Yang, Zhao Yongfang, Liu Feng, Chen Nansheng

机构信息

CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.

Laboratory of Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.

出版信息

Mitochondrial DNA B Resour. 2021 Jun 3;6(7):1849-1851. doi: 10.1080/23802359.2021.1934578.

Abstract

is a species-rich genus with about 400 species described, many of which are harmful algal bloom species with significant negative ecological impact. Despite of their importance in primary production and as harmful algal bloom species, genome data for species in this genus is limited. No mitochondrial genome (mtDNA) of any species in this genus has been reported. Here, the complete mtDNA sequence of the Gran & Angst 1931 was constructed and analyzed. The circular mtDNA was 36,071 bp in length, encoding 64 genes, including 34 protein coding genes (PCGs), 24 transfer RNA (tRNA) genes, 2 ribosomal RNA (rRNA) genes and 4 conserved open reading frames (s). The overall AT content of mtDNA was 75.00%, which was slightly lower than that of (78.40%). Maximum likelihood (ML) phylogenetic analysis using 29 shared protein-coding genes revealed that clustered well with , which was the only species of class Coscinodiscophyceae whose mtDNA has been fully constructed. The complete mtDNAs of more species will be valuable for studying the evolutionary relationships among species in the genus and in the Class of Coscinodiscophyceae.

摘要

是一个物种丰富的属,已描述约400个物种,其中许多是具有重大负面生态影响的有害藻华物种。尽管它们在初级生产中以及作为有害藻华物种很重要,但该属物种的基因组数据有限。该属中任何物种的线粒体基因组(mtDNA)都尚未见报道。在此,构建并分析了1931年格兰和安斯特所描述物种的完整mtDNA序列。环状mtDNA长度为36,071 bp,编码64个基因,包括34个蛋白质编码基因(PCGs)、24个转运RNA(tRNA)基因、2个核糖体RNA(rRNA)基因和4个保守开放阅读框(s)。mtDNA的总体AT含量为75.00%,略低于(78.40%)。使用29个共享蛋白质编码基因进行的最大似然(ML)系统发育分析表明,与 聚类良好, 是硅藻纲中唯一其mtDNA已被完全构建的物种。更多物种的完整mtDNA对于研究该属以及硅藻纲物种之间的进化关系将具有重要价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/780c/8183543/b7cd49724891/TMDN_A_1934578_F0001_C.jpg

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