miR-92a-3p 通过调控 PI3K/AKT/mTOR 信号通路促进肝癌的恶性进展。
MiR-92a-3p Promotes the Malignant Progression of Hepatocellular Carcinoma by Mediating the PI3K/AKT/mTOR Signaling Pathway.
机构信息
Department of Hepatobiliary Surgery, Tangshan Gongren Hospital, Tangshan, 063000, China.
Shanghai Engineering Research Center of Pharmaceutical Translation, 200231, Shanghai, China.
出版信息
Curr Pharm Des. 2021;27(29):3244-3250. doi: 10.2174/1381612827666210612054156.
BACKGROUND
As one of the most common cancers globally, hepatocellular carcinoma (HCC) usually has a poor prognosis. Many HCC patients are usually diagnosed at advanced stages. Therefore, new potential biomarkers for the diagnosis and prognosis of HCC are urgently needed. More and more studies have shown that miR-92a-3p can regulate the occurrence and development of various cancers, but its clinical significance and molecular mechanism in HCC are still elusive. Here, we tried to clarify the regulatory mechanism of miR-92a-3p in HCC.
METHODS
In this study, we conducted qRT-PCR and revealed that miR-92a-3p was notably upregulated in HCC cells. MTT, flow cytometry, wound healing, Transwell invasion assays and western blot were conducted to uncover that overexpressed miR-92a-3p could boost the proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) of HCC cells while inhibiting cell apoptosis. In addition, the proteins associated with the PI3K/AKT/mTOR pathway were also detected by western blot.
RESULTS
It was suggested that miR-92a-3p could activate the PI3K/AKT/mTOR signaling pathway.
CONCLUSION
These results suggest that miR-92a-3p plays a tumor-promoting role in HCC and may be a potential biomarker for the diagnosis and prognosis of HCC.
背景
作为全球最常见的癌症之一，肝细胞癌（HCC）通常预后较差。许多 HCC 患者通常在晚期被诊断出来。因此，迫切需要新的潜在生物标志物来诊断和预测 HCC。越来越多的研究表明，miR-92a-3p 可以调节各种癌症的发生和发展，但它在 HCC 中的临床意义和分子机制仍不清楚。在这里，我们试图阐明 miR-92a-3p 在 HCC 中的调控机制。
方法
在本研究中，我们通过 qRT-PCR 发现 miR-92a-3p 在 HCC 细胞中显著上调。通过 MTT、流式细胞术、划痕愈合、Transwell 侵袭实验和 Western blot 揭示，过表达 miR-92a-3p 可以促进 HCC 细胞的增殖、迁移、侵袭和上皮-间充质转化（EMT），同时抑制细胞凋亡。此外，还通过 Western blot 检测了与 PI3K/AKT/mTOR 通路相关的蛋白。
结果
表明 miR-92a-3p 可以激活 PI3K/AKT/mTOR 信号通路。
结论
这些结果表明，miR-92a-3p 在 HCC 中发挥促肿瘤作用，可能是 HCC 诊断和预后的潜在生物标志物。
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