Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zurich, Basel, Switzerland.
J Exp Med. 2021 Jul 5;218(7). doi: 10.1084/jem.20201546. Epub 2021 Jun 15.
Cells constantly sense their environment, allowing the adaption of cell behavior to changing needs. Fine-tuned responses to complex inputs are computed by signaling pathways, which are wired in complex connected networks. Their activity is highly context-dependent, dynamic, and heterogeneous even between closely related individual cells. Despite lots of progress, our understanding of the precise implementation, relevance, and possible manipulation of cellular signaling in health and disease therefore remains limited. Here, we discuss the requirements, potential, and limitations of the different current technologies for the analysis of hematopoietic stem and progenitor cell signaling and its effect on cell fates.
细胞不断感知其环境，从而使细胞行为适应不断变化的需求。通过信号通路来计算对复杂输入的精细响应，这些信号通路在复杂的互联网络中布线。即使在密切相关的个体细胞之间，它们的活性也高度依赖于上下文、动态且异质。尽管取得了很多进展，但我们对细胞信号在健康和疾病中的精确实施、相关性和可能的操纵的理解仍然有限。在这里，我们讨论了分析造血干细胞和祖细胞信号及其对细胞命运影响的不同现有技术的要求、潜力和局限性。
