Saraste J, Bronson M, Palade G E, Farquhar M G
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
Prog Clin Biol Res. 1988;270:129-39.
We have devised a strategy for producing Golgi subcompartment-specific antibodies that involves utilizing Triton X-114-phase separated membrane proteins derived from rat pancreatic Golgi subfractions as immunogens. When we tested the approach by immunizing rabbits with membrane proteins derived from heavy Golgi subfractions that are known to be enriched in cis Golgi elements, we succeeded in generating an antibody that recognized a 58 kD protein that was restricted in its distribution to cis Golgi cisternae in several cell types. Thus we have demonstrated the feasibility of the approach we devised for generation of Golgi subcompartment-specific antibodies, and we have also succeeded in identifying a heretofore unknown cis Golgi marker protein.
我们设计了一种生产高尔基体亚区特异性抗体的策略，该策略涉及利用源自大鼠胰腺高尔基体亚组分的Triton X-114相分离膜蛋白作为免疫原。当我们用源自已知富含顺面高尔基体元件的重高尔基体亚组分的膜蛋白免疫兔子来测试该方法时，我们成功产生了一种抗体，该抗体识别一种58 kD的蛋白质，其分布局限于几种细胞类型的顺面高尔基体潴泡。因此，我们证明了我们设计的用于生成高尔基体亚区特异性抗体的方法的可行性，并且我们还成功鉴定了一种迄今为止未知的顺面高尔基体标记蛋白。
