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使用含S层蛋白的乳极性脂质体优化包封及ACE抑制肽RLSFNP的转运研究

Optimization of Encapsulation Using Milk Polar Lipid Liposomes with S-Layer Protein and Transport Study of the ACE-Inhibitory Peptide RLSFNP.

作者信息

Zhang Tao, Su Mi, Liu Mingzhen, Tao Mingxuan, Yang Yao, Liu Chen, Zeng Xiaoqun, Pan Daodong, Wu Zhen, Guo Yuxing

机构信息

Department of Food Science and Technology, School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing 210097, Jiangsu, P. R. China.

State Key Laboratory for Quality and Safety of Agro-products, Ningbo University, Ningbo 315211, Zhejiang, P. R. China.

出版信息

J Agric Food Chem. 2021 Jun 16. doi: 10.1021/acs.jafc.1c02216.

Abstract

The purpose of this study is to develop a new type of nanodrug delivery material by modifying milk polar lipid (MPL) liposomes with the S-layer protein. LIP-RLSFNP (MPL liposomes encapsulating RLSFNP (Arg-Leu-Ser-Phe-Asn-Pro)) and SLP-LIP-RLSFNP (S-layer protein-modified LIP-RLSFNP) were prepared and characterized by transmission electron microscopy, Fourier transform infrared spectroscopy, confocal laser scanning microscopy, surface plasmon resonance, and mastersizer dynamic light scattering measurements. The results showed that the S-layer protein could modify the surface of MPL liposomes, stabilize the shape of the vesicles, and improve the resistance to external interference. Furthermore, SLP-LIP-RLSFNP showed better performance in and experiments compared with LIP-RLSFNP in terms of promoting absorption and delayed release. The findings suggested that MPL liposomes modified with the S-layer protein have potential for use as an effective delivery system for therapeutic proteins and peptides.

摘要

本研究的目的是通过用S层蛋白修饰乳极性脂质(MPL)脂质体来开发一种新型纳米药物递送材料。制备了LIP-RLSFNP(包裹RLSFNP(精氨酸-亮氨酸-丝氨酸-苯丙氨酸-天冬酰胺-脯氨酸)的MPL脂质体)和SLP-LIP-RLSFNP(S层蛋白修饰的LIP-RLSFNP),并通过透射电子显微镜、傅里叶变换红外光谱、共聚焦激光扫描显微镜、表面等离子体共振和激光粒度分析仪动态光散射测量对其进行了表征。结果表明,S层蛋白可以修饰MPL脂质体的表面,稳定囊泡的形状,并提高对外部干扰的抗性。此外,在促进吸收和缓释方面,与LIP-RLSFNP相比,SLP-LIP-RLSFNP在[具体实验名称1]和[具体实验名称2]实验中表现出更好的性能。研究结果表明,用S层蛋白修饰的MPL脂质体有潜力用作治疗性蛋白质和肽的有效递送系统。

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