College of Chemistry and Material Science, Hebei Key Laboratory of Organic Functional Molecules, Hebei Normal University, Shijiazhuang, 050024, China.
Department of Rehabilitation Medicine, Shijiazhuang People's Hospital, Shijiazhuang, 050000, China.
Appl Biochem Biotechnol. 2023 Oct;195(10):5950-5965. doi: 10.1007/s12010-023-04344-w. Epub 2023 Feb 2.
Nano-resveratrol liposome (RES-LIP) was prepared by the thin film rotary-evaporated method combined with ultrasonication and characterized by transmission electron microscopy (TEM), zeta potential, dynamic light scattering (DLS), and Fourier-transform infrared (FT-IR). The physicochemical stability, in vitro release, antioxidant activity, and cytotoxicity of RES-LIP were studied. Data showed that RES-LIP was a spherical vesicle with a diameter of less than 100 nm, the zeta potential was - 60 mV and the encapsulation efficiency was 86.78%. The physicochemical stability of RES-LIP was determined by E, ΔG, ΔH, and ΔS, which suggested that the process of RES-LIP degradation was spontaneous and endothermic. The in vitro release of RES-LIP was pH-dependent, belonged to the Weibull model, and was non-Fick diffusion. The antioxidant activity of RES-LIP was stronger than free resveratrol. The MTT assay and flow cytometry results suggested that resveratrol decreased cytotoxicity after being encapsulated by liposome. The prepared RES-LIP had high encapsulation efficiency, was sustained-release, had low cytotoxicity, was pH-targeted, and had potential usage in food and medicine fields.
纳米白藜芦醇脂质体（RES-LIP）采用薄膜旋转蒸发法结合超声法制备，采用透射电子显微镜（TEM）、Zeta 电位、动态光散射（DLS）和傅里叶变换红外（FT-IR）进行表征。研究了 RES-LIP 的理化稳定性、体外释放、抗氧化活性和细胞毒性。结果表明，RES-LIP 为粒径小于 100nm 的球形囊泡，Zeta 电位为-60mV，包封率为 86.78%。通过 E、ΔG、ΔH 和 ΔS 确定了 RES-LIP 的理化稳定性，表明 RES-LIP 降解过程是自发的和吸热的。RES-LIP 的体外释放呈 pH 依赖性，属于 Weibull 模型，是非 Fick 扩散。RES-LIP 的抗氧化活性强于游离白藜芦醇。MTT 检测和流式细胞术结果表明，脂质体包封白藜芦醇后降低了细胞毒性。所制备的 RES-LIP 具有较高的包封效率、缓释性、低细胞毒性、pH 靶向性，在食品和医药领域具有潜在的应用价值。
