Department of Bioinformatics and Biostatistics, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
J Cell Biochem. 2021 Oct;122(10):1428-1434. doi: 10.1002/jcb.30019. Epub 2021 Jun 16.
Interpreting functional analysis results derived from environmental samples using direct sequencing meta-omics data, including metagenomics and meta-transcriptomics data, is challenging due to their complexity. Visualization of functional analysis results can help researchers discover relevant biological insights. Despite the availability of many R packages, there lacks interactive and comprehensive graphic systems for displaying functional terms and corresponding genes in meta-omics analysis results. Here, we present ivTerm, an R-shiny package with a user-friendly graphical interface that enables users to inspect functional annotations, compare results across multiple experiments, create customized charts, and download these charts. It provides various basic and innovative chart types to visualize functional terms and involved genes. Users can also browse the description of terms obtained from the database web servers automatically. Two examples, including a metagenome analysis data for human gut and a meta-transcriptome data for coral symbiomes, are given to show the usage of ivTerm. In the end, we compared ivTerm with existing tools with similar functions, such as GOplot, ViSEAGO, and Chordomics. The tool ivTerm is convenient and efficient for biologists to gain an integrated view and develop deep insights by interactive analysis of meta-omics data. It can accelerate the procedure to develop insights from complex meta-omics data. The code for ivTerm is freely available at https://github.com/SJTU-CGM/ivTerm.
由于其复杂性,使用直接测序宏基因组学数据(包括宏基因组学和宏转录组学数据)来解释从环境样本中得出的功能分析结果具有挑战性。功能分析结果的可视化可以帮助研究人员发现相关的生物学见解。尽管有许多 R 包可用,但缺乏用于显示宏基因组学分析结果中功能术语和相应基因的交互式和综合图形系统。在这里,我们介绍了 ivTerm,这是一个带有用户友好图形界面的 R-shiny 包,使用户能够检查功能注释、比较多个实验的结果、创建自定义图表以及下载这些图表。它提供了各种基本和创新的图表类型来可视化功能术语和涉及的基因。用户还可以自动浏览从数据库 Web 服务器中获取的术语描述。提供了两个示例,包括人类肠道的宏基因组分析数据和珊瑚共生体的元转录组数据,以展示 ivTerm 的用法。最后,我们将 ivTerm 与具有类似功能的现有工具(如 GOplot、ViSEAGO 和 Chordomics)进行了比较。ivTerm 工具对于生物学家来说非常方便和高效,可以通过交互式分析宏基因组学数据来获得综合视图并深入了解。它可以加速从复杂的宏基因组学数据中获得见解的过程。ivTerm 的代码可在 https://github.com/SJTU-CGM/ivTerm 上免费获得。
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