Shvachko L P, Kibirev V K
Ukr Biokhim Zh (1978). 1988 May-Jun;60(3):15-9.
Isopropanol is shown to affect considerably the thrombin activity. Its low concentrations (5-15%) activate the hydrolysis reaction of benzoyl-1-arginine ethyl ester (BAEE) by thrombin, whereas incomplete uncompetitive inhibition of the enzyme is observed in the presence of 20% isopropanol. Alcohol in the concentration of 25% results in complete reversible inhibition of the clothing and esterase activity of the enzyme. Isopropanol in the concentration of 25% is able to suppress the thrombin autolysis and therefore it may be used as a reagent which stabilizes thrombin during its storage in the aquatic-salt solutions.
已证明异丙醇对凝血酶活性有相当大的影响。其低浓度（5 - 15%）能激活凝血酶对苯甲酰 - 1 - 精氨酸乙酯（BAEE）的水解反应，而在20%异丙醇存在下观察到该酶存在不完全非竞争性抑制。25%浓度的酒精会导致该酶的凝血和酯酶活性完全可逆抑制。25%浓度的异丙醇能够抑制凝血酶自溶，因此它可作为一种在凝血酶储存于水盐溶液期间使其稳定的试剂。
