Department of Animal Health and Management, Alagappa University, Karaikudi, 630 003, Tamilnadu, India.
Department of Zoology, University of Madras, Guindy Campus, Chennai, 600 025, India.
Chemosphere. 2021 Nov;283:130979. doi: 10.1016/j.chemosphere.2021.130979. Epub 2021 Jun 1.
The present study has investigated the distribution of microplastics in sediment and its impact on histological, ultrastructural, and oxidative stress mechanisms in Perna viridis (P. viridis) from Kasimedu, Chennai, India. The results confirmed that fibers were the predominant type of microplastics observed, followed by spheres, flakes, sheets, and fragments. The observed microplastics were confirmed as polyester, polypropylene, polyethylene, cellophane, and rayon using μ-FT-IR. Microplastic particles entangled in gills caused abrasion of ciliated structure and hemocyte infiltration in the hemolymph vessels. The digestive gland showed a shrunken nucleus, dark inclusions, and damage in the nucleoid core structure. Enlarged vacuoles and the presence of clusters of vesicles presumably represented the transformed golgi cisternae. Further, the results confirmed that oxidative stress markers were significantly high in gills and digestive diverticula of P. viridis. Overall, the results indicated that microplastics induced different toxic physiological and structural alterations in gills and digestive diverticula of P. viridis. These findings highlighted the necessity to focus on exposure studies to understand the absolute magnitude of the problem due to microplastic pollution in the urban estuarine ecosystems of Chennai, Tamil Nadu, India.
本研究调查了微塑料在沉积物中的分布及其对印度钦奈卡西梅杜的青口贝（Perna viridis，P. viridis）组织学、超微结构和氧化应激机制的影响。结果证实，纤维是观察到的主要类型的微塑料，其次是球体、薄片、薄片和碎片。使用 μ-FT-IR 确认观察到的微塑料为聚酯、聚丙烯、聚乙烯、赛璐珞和人造丝。缠绕在鳃上的微塑料颗粒导致纤毛结构磨损和血细胞渗透到血液血管中。消化腺显示细胞核缩小、深色内含物和核基质核心结构受损。扩大的空泡和囊泡簇的存在可能代表转化的高尔基内质网。此外，结果证实 P. viridis 的鳃和消化憩室中的氧化应激标志物显着升高。总体而言，结果表明微塑料在 P. viridis 的鳃和消化憩室中引起了不同的毒性生理和结构改变。这些发现强调了需要关注暴露研究，以了解由于印度泰米尔纳德邦钦奈城市河口生态系统中的微塑料污染而导致的问题的绝对规模。
