不同年龄男性腹股沟疝患者的基质金属蛋白酶（MMP-2）和金属蛋白酶组织抑制剂（TIMP-2）。

Matrix Metalloproteinases (MMP-2) and Tissue Inhibitors of Metalloproteinases (TIMP-2) in Male Inguinal Hernia Patients at Different Ages.

机构信息

Department of Hernia and Abdominal Wall Surgery, The First Affiliated Hospital of Zhengzhou University, No.1 Jianshe Road, Zhengzhou, 450052, Henan, China.

Department of General Surgery, The First People's Hospital of Jiande City, No.599 Yanzhou Road, Jiande, 311600, Zhejiang, China.

出版信息

World J Surg. 2021 Sep;45(9):2747-2751. doi: 10.1007/s00268-021-06198-x. Epub 2021 Jun 21.

DOI:10.1007/s00268-021-06198-x

PMID:34155539

Abstract

BACKGROUND

Collagen metabolism, controlled by matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs), might be related to inguinal hernia formation. It was reported that the incidence of inguinal hernia and the recurrence rate after inguinal hernia repair were higher in the elderly. The objective of the research was to assess the amounts of MMP-2 and TIMP-2 in patients at different ages in order to examine the relationship between age and inguinal hernia occurrence.

METHODS

The research included 40 primary inguinal hernia male patients, and four groups were created: 50-59 years old (A group); 60-69 years old (B group); 70-79 years old (C group); 80-89 years old (D group). We got the samples from anterior rectus sheath fascia. Real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and immunohistochemistry were applied to estimate the levels of MMP-2 and TIMP-2.

RESULTS

The MMP-2 amounts in C and D group were statistically higher than control group (P < 0.05), and the TIMP-2 levels in C and D group were statistically lower than control group (P < 0.05). We found a positive correlation between age and expression levels of MMP-2 (r = 0.537, P < 0.001; r = 0.569, P < 0.001) and a negative correlation between age and TIMP-2 in inguinal hernia patients (r = - 0.759, P < 0.001; r = - 0.759, P < 0.001).

CONCLUSIONS

Increased MMP-2 and reduced TIMP-2 may have some relationships with higher inguinal hernia incidence of the elderly.

摘要

背景

胶原代谢受基质金属蛋白酶（MMPs）和金属蛋白酶组织抑制剂（TIMPs）的控制，可能与腹股沟疝的形成有关。有报道称，老年人的腹股沟疝发病率和疝修补术后复发率较高。本研究的目的是评估不同年龄患者 MMP-2 和 TIMP-2 的含量，以探讨年龄与腹股沟疝发生的关系。

方法

本研究纳入了 40 例原发性腹股沟疝男性患者，分为 4 组：50-59 岁（A 组）；60-69 岁（B 组）；70-79 岁（C 组）；80-89 岁（D 组）。我们从前腹壁直肌鞘筋膜获取样本。应用实时荧光定量聚合酶链反应（RT-PCR）和免疫组织化学法评估 MMP-2 和 TIMP-2 的水平。

结果

C 组和 D 组 MMP-2 含量明显高于对照组（P<0.05），C 组和 D 组 TIMP-2 水平明显低于对照组（P<0.05）。我们发现年龄与 MMP-2 的表达水平呈正相关（r=0.537，P<0.001；r=0.569，P<0.001），与腹股沟疝患者 TIMP-2 呈负相关（r=-0.759，P<0.001；r=-0.759，P<0.001）。