人肝环氧化物水解酶在粟酒裂殖酵母中的表达。
Expression of human liver epoxide hydrolase in Saccharomyces pombe.
作者信息
Jackson M R, Burchell B
机构信息
Department of Biochemistry, The University, Scotland, U.K.
出版信息
Biochem J. 1988 May 1;251(3):931-3. doi: 10.1042/bj2510931.
Abstract
Human liver microsomal epoxide hydrolase cDNA was inserted into the yeast expression vector pEVP11. The resulting recombinant plasmid was introduced into Saccharomyces pombe. The epoxide hydrolase protein and enzymic activity was subsequently expressed and identified in the 105,000 g pellet after centrifugal fractionation of homogenized yeast cells. This method will provide a useful source of human liver epoxide hydrolase, avoiding the problems of obtaining human tissue.
摘要
将人肝微粒体环氧化物水解酶cDNA插入酵母表达载体pEVP11中。将所得重组质粒导入粟酒裂殖酵母。随后，在对匀浆酵母细胞进行离心分级分离后，在105,000 g沉淀中表达并鉴定了环氧化物水解酶蛋白和酶活性。该方法将提供一种有用的人肝环氧化物水解酶来源，避免了获取人体组织的问题。
相似文献
1
Expression of human liver epoxide hydrolase in Saccharomyces pombe.人肝环氧化物水解酶在粟酒裂殖酵母中的表达。
Biochem J. 1988 May 1;251(3):931-3. doi: 10.1042/bj2510931.
2
Heterologous expression of human microsomal epoxide hydrolase in Saccharomyces cerevisiae. Study of the valpromide-carbamazepine epoxide interaction.人微粒体环氧化物水解酶在酿酒酵母中的异源表达。丙戊酰胺 - 卡马西平环氧化物相互作用的研究。
Biochem Pharmacol. 1991 Sep 12;42(7):1367-72. doi: 10.1016/0006-2952(91)90447-d.
3
cDNA cloning and expression of a soluble epoxide hydrolase from human liver.
Arch Biochem Biophys. 1993 Aug 15;305(1):197-201. doi: 10.1006/abbi.1993.1411.
4
Molecular cloning and expression of murine liver soluble epoxide hydrolase.
J Biol Chem. 1993 Aug 15;268(23):17628-33.
5
Mechanism of action and cloning of epoxide hydrolase from the cabbage looper, Trichoplusia ni.甘蓝夜蛾（Trichoplusia ni）环氧化物水解酶的作用机制及克隆
Arch Insect Biochem Physiol. 1996;32(3-4):527-35. doi: 10.1002/(SICI)1520-6327(1996)32:3/4<527::AID-ARCH24>3.0.CO;2-D.
6
Effect of phenobarbital on the level of translatable rat liver epoxide hydrolase mRNA.苯巴比妥对大鼠肝脏可翻译的环氧化物水解酶mRNA水平的影响。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):893-7. doi: 10.1073/pnas.78.2.893.
7
Expression of human leukotriene A4 hydrolase cDNA in Escherichia coli.人白三烯A4水解酶cDNA在大肠杆菌中的表达。
FEBS Lett. 1988 Mar 14;229(2):279-82. doi: 10.1016/0014-5793(88)81140-2.
8
9
[Comparative characteristics of microsomal epoxide hydrolase in the rat liver and small intestine mucosa].[大鼠肝脏和小肠黏膜中微粒体环氧化物水解酶的比较特征]
Vopr Med Khim. 1988 Nov-Dec;34(6):122-8.
10
Characterization of a Euphorbia lagascae epoxide hydrolase gene that is induced early during germination.大戟科拉加斯卡大戟环氧水解酶基因在萌发早期的诱导特性。
Biochem Soc Trans. 2000 Dec;28(6):855-7.
引用本文的文献
1
Diversity and biocatalytic potential of epoxide hydrolases identified by genome analysis.通过基因组分析鉴定的环氧水解酶的多样性及生物催化潜力
Appl Environ Microbiol. 2006 Apr;72(4):2905-17. doi: 10.1128/AEM.72.4.2905-2917.2006.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.组织分级分离研究。6. 大鼠肝脏组织中酶的细胞内分布模式。
Biochem J. 1955 Aug;60(4):604-17. doi: 10.1042/bj0600604.
3
Molecular properties and biological functions of microsomal epoxide hydrase.微粒体环氧化物水解酶的分子特性与生物学功能
Annu Rev Pharmacol Toxicol. 1980;20:513-31. doi: 10.1146/annurev.pa.20.040180.002501.
4
Drug detoxification: epoxide hydrolase.药物解毒：环氧水解酶。
Prog Clin Biol Res. 1983;135:81-105.
5
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
6
Purification and specificity of a human microsomal epoxide hydratase.人微粒体环氧化物水解酶的纯化及特异性
Biochem J. 1974 Apr;139(1):77-88. doi: 10.1042/bj1390077.
7
Epoxide hydrolase is a marker for the smooth endoplasmic reticulum in rat liver.环氧水解酶是大鼠肝脏中滑面内质网的标志物。
EMBO J. 1985 Nov;4(11):2793-800. doi: 10.1002/j.1460-2075.1985.tb04005.x.
8
Complementary DNA and amino acid sequence of rat liver microsomal, xenobiotic epoxide hydrolase.大鼠肝脏微粒体异生物质环氧化物水解酶的互补DNA及氨基酸序列
Arch Biochem Biophys. 1986 Jul;248(1):121-9. doi: 10.1016/0003-9861(86)90408-x.
9
Nucleotide and deduced amino acid sequence of human liver microsomal epoxide hydrolase.人肝脏微粒体环氧化物水解酶的核苷酸及推导的氨基酸序列
Nucleic Acids Res. 1987 Sep 11;15(17):7188. doi: 10.1093/nar/15.17.7188.
10
The mitotic inducer nim1+ functions in a regulatory network of protein kinase homologs controlling the initiation of mitosis.有丝分裂诱导剂nim1 +在控制有丝分裂起始的蛋白激酶同源物调控网络中发挥作用。
Cell. 1987 May 22;49(4):569-76. doi: 10.1016/0092-8674(87)90459-4.
Zotero 插件