Key Laboratory of Coarse Cereal Processing, Ministry of Agriculture and Rural 7 Affairs, School of Food and Biological Engineering, Chengdu University, Chengdu 8610106, PR China.
Department of Pharmaceutical and Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
J Chromatogr A. 2021 Aug 16;1651:462337. doi: 10.1016/j.chroma.2021.462337. Epub 2021 Jun 9.
Here, a m-xylene bisphosphonate immobilized tentacle-type cellulose monolith (BP-PCM) is prepared by atom transfer radical polymerization for lysozyme purification. In the preparation, the m-xylene bisphosphonate was anchored glycidyl methacrylate and then polymerized to enhance the flexibility of the ligands to improve lysozyme adsorption capacity, and glycerol monomethacrylate serves as spacer to further optimize the layers structure and ligands density of the grafted tentacles for satisfactory adsorption capacity. The maximum static and dynamic adsorption capacity (10% breakthrough) of BP-PCM reach to 169.6 and 102.6 mg mL, respectively. Moreover, BP-PCM displays weak nonspecific adsorption and is able to successfully enrich lysozyme from diluted chicken egg white, indicating the excellent selectivity. The results demonstrated that BP-PCM is promising for use as high-capacity protein chromatography.
在这里，通过原子转移自由基聚合制备了一种固定化在触角型纤维素整体柱上的间二甲苯双膦酸（BP-PCM），用于溶菌酶的纯化。在制备过程中，间二甲苯双膦酸被锚定到甲基丙烯酰氧乙基磷酸酯上，然后聚合以增强配体的柔韧性，提高溶菌酶的吸附容量，而甲基丙烯酸甘油酯则作为间隔物，进一步优化接枝触角的层状结构和配体密度，以获得令人满意的吸附容量。BP-PCM 的最大静态和动态吸附容量（10%穿透）分别达到 169.6 和 102.6mg/mL。此外，BP-PCM 表现出较弱的非特异性吸附能力，并能够成功地从稀释的鸡蛋白中富集溶菌酶，显示出优异的选择性。结果表明，BP-PCM 有望用作大容量蛋白质色谱。
