UMR9190-MARBEC, Université de Montpellier, Place Eugène Bataillon, 34095, Montpellier, France.
Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, 27570, Bremerhaven, Germany.
Environ Sci Pollut Res Int. 2021 Nov;28(43):60649-60662. doi: 10.1007/s11356-021-14892-5. Epub 2021 Jun 23.
Mangrove crabs are ecosystem engineers through their bioturbation activity. On Mayotte Island, the abundance of Neosarmatium africanum decreased in wastewater-impacted areas. Previous analyses showed that global crab metabolism is impacted by wastewater, with a burst in O consumption that may be caused by osmo-respiratory trade-offs since gill functioning was impacted. As the hepatopancreas is a key metabolic organ, the purpose of this study was to investigate the physiological effects of wastewater and ammonia-N 5-h exposure on crabs to better understand the potential trade-offs underlying the global metabolic state. Catalase, superoxide dismutase, glutathione S-transferase, total digestive protease, and serine protease (trypsin and chymotrypsin) activities were assessed. Histological analyses were performed to determine structural modifications. No effect of short-term wastewater and ammonia-N exposure was found in antioxidant defenses or digestive enzyme activity. However, histological changes of B-cells indicate an increase in intracellular digestive activity through higher vacuolization processes and tubule dilation in wastewater-exposed crabs.
红树林蟹通过其生物搅动活动成为生态系统工程师。在马约特岛，非洲新沙蟹的丰度在受废水影响的地区减少。先前的分析表明，全球螃蟹代谢受到废水的影响，由于鳃功能受到影响，O 消耗爆发，这可能是由渗透呼吸权衡引起的。由于肝胰腺是一个关键的代谢器官，本研究的目的是研究废水和氨氮 5-h 暴露对螃蟹的生理影响，以更好地了解全球代谢状态下潜在的权衡。评估了过氧化氢酶、超氧化物歧化酶、谷胱甘肽 S-转移酶、总消化蛋白酶和丝氨酸蛋白酶（胰蛋白酶和糜蛋白酶）的活性。进行了组织学分析以确定结构修饰。短期废水和氨氮暴露对抗氧化防御或消化酶活性没有影响。然而，B 细胞的组织学变化表明，通过更高的空泡化过程和废水暴露螃蟹中管腔扩张，细胞内消化活性增加。
