Department of General Surgery, Beibei Traditional Chinese Medical Hospital, Fengdu County, Chongqing, China.
Department of General Surgery, Fengdu People's Hospital, Fengdu County, Chongqing, China
Ann Clin Lab Sci. 2021 May;51(3):339-346.
To investigate the role of circular RNA MYLK (circMYLK) in colorectal cancer (CRC).
Patients with CRC were enrolled and cancerous tissues were collected. CircMYLK expression was measured by quantitative real-time polymerase chain reaction (qRT-PCR). The diagnostic value of circMYLK was determined by ROC curve. In vitro knockdown of circMYLK was carried out to investigate the proliferation, migration and invasion capabilities of CRC cell lines.
We first found that circMYLK expression was markedly enhanced in human CRC tissues, serum and cell lines, which was correlated with poor prognosis and some detrimental clinical characteristics. ROC curve showed that circMYLK could be a diagnostic biomarker of CRC. The proliferation ability of CRC cells was markedly inhibited, while higher apoptosis rate was induced after circMYLK deplition. In addition, the migration and invasion abilities of CRC cells were suppressed following circMYLK silencing.
Our results implicate that circMYLK functions as oncogenic stimuli in CRC and may be a novel diagnostic and therapeutic targets of CRC.
研究肌球蛋白轻链（MYLK）环状 RNA（circMYLK）在结直肠癌（CRC）中的作用。
纳入 CRC 患者并收集癌组织。通过实时定量聚合酶链反应（qRT-PCR）测定 circMYLK 的表达。通过 ROC 曲线确定 circMYLK 的诊断价值。体外敲低 circMYLK 以研究 CRC 细胞系的增殖、迁移和侵袭能力。
我们首先发现 circMYLK 在人 CRC 组织、血清和细胞系中的表达明显增强，与不良预后和一些不良临床特征相关。ROC 曲线表明 circMYLK 可作为 CRC 的诊断生物标志物。CRC 细胞的增殖能力明显受到抑制，而 circMYLK 耗尽后则诱导更高的细胞凋亡率。此外，circMYLK 沉默抑制了 CRC 细胞的迁移和侵袭能力。
我们的研究结果表明，circMYLK 在 CRC 中发挥致癌刺激作用，可能是 CRC 的一种新的诊断和治疗靶点。
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