Chen Dongfei, Wu Yanfang, Hoque Sharmin, Tilley Richard D, Gooding J Justin
School of Chemistry, The University of New South Wales Sydney NSW 2052 Australia
Australian Centre for NanoMedicine, The University of New South Wales Sydney NSW 2052 Australia.
Chem Sci. 2021 Mar 3;12(14):5196-5201. doi: 10.1039/d1sc01044a.
An accurate and robust method for quantifying the levels of circulating tumor DNA (ctDNA) is vital if this potential biomarker is to be used for the early diagnosis of cancer. The analysis of ctDNA presents unique challenges because of its short half-life and ultralow abundance in early stage cancers. Here we develop an ultrasensitive electrochemical biosensor for rapid detection of ctDNA in whole blood. The sensing of ctDNA is based on hybridization on a network of probe DNA modified gold-coated magnetic nanoparticles (DNA-Au@MNPs). This DNA-Au@MNPs biosensor can selectively detect short- and long-strand DNA targets. It has a broad dynamic range (2 aM to 20 nM) for 22 nucleotide DNA target with an ultralow detection limit of 3.3 aM. For 101 nucleotide ctDNA target, a dynamic range from 200 aM to 20 nM was achieved with a detection limit of 5 fM. This DNA-Au@MNPs based sensor provides a promising method to achieve 20 min response time and minimally invasive cancer early diagnosis.
如果要将这种潜在的生物标志物用于癌症的早期诊断，那么一种准确且可靠的循环肿瘤DNA（ctDNA）定量方法至关重要。由于ctDNA半衰期短且在早期癌症中丰度超低，其分析面临独特挑战。在此，我们开发了一种超灵敏电化学生物传感器，用于快速检测全血中的ctDNA。ctDNA的传感基于在探针DNA修饰的金包被磁性纳米颗粒（DNA-Au@MNPs）网络上的杂交。这种DNA-Au@MNPs生物传感器能够选择性地检测短链和长链DNA靶标。对于22个核苷酸的DNA靶标，它具有宽泛的动态范围（2 aM至20 nM），超低检测限为3.3 aM。对于101个核苷酸的ctDNA靶标，实现了200 aM至20 nM的动态范围，检测限为5 fM。这种基于DNA-Au@MNPs的传感器为实现20分钟响应时间和微创癌症早期诊断提供了一种有前景的方法。
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