College of Animal Science and Technology, Henan Agricultural University, Zhengzhou, China.
Henan Innovative Engineering Research Center of Poultry Germplasm Resource, Zhengzhou, China.
Mol Omics. 2021 Oct 11;17(5):752-759. doi: 10.1039/d1mo00011j.
Valgus-varus Deformity (VVD) leg disease often affects chickens raised in modern large-scale breeding operations. Losses due to VVD are costly to farmers, and the condition also causes significant suffering in affected birds. In this study, we profiled RNAs from the spleens of VVD (BS) and healthy (JS) broilers using high-throughput sequencing to identify miRNAs that might be involved in the development of the disease. Fifty differentially expressed miRNAs (DEMs) were found, of which 30 were up-regulated and 20 were down-regulated in VVD-affected birds (|log 2 Fold Change| ≥ 1 and -value < 0.05). DEMs were matched with putative target genes and 864 target genes were found. Gene Ontology (GO) analyses of these target genes showed that they were significantly enriched in the "cytoplasm" term (-value < 0.05), and most of the target genes were enriched in "cellular component". Kyoto encyclopedia of genes and genomes (KEGG) analysis showed that they were significantly enriched in 11 signaling pathways (-value < 0.05), including metabolic pathways, 2-oxocarboxylic acid metabolism, regulation of actin cytoskeleton, purine metabolism, endocytosis and so on. And we found that they were enriched in immune-related pathways in which MAPK, Notch, JAK-Stat, Toll-like receptor, p53 and other single pathways were involved in the development of skeletal diseases. Differentially expressed mRNAs obtained from our previous study were used to construct an interaction network consisting of 16 DEMs and 21 differentially expressed mRNAs (|log 2 Fold Change| ≥ 1 and -value < 0.05). We found that miR-12247-5p, miR-15c-5p, miR-15b-5p, and were at the center of network interaction. This study provides a foundation for further investigations of the pathogenesis and genetic mechanisms underlying VVD.
瓦氏变形（VVD）腿部疾病常影响现代大规模养殖操作中的鸡。VVD 给农民造成的损失是巨大的，而且这种疾病也会给受影响的鸟类带来严重的痛苦。在这项研究中，我们使用高通量测序对 VVD（BS）和健康（JS）肉鸡的脾脏中的 RNA 进行了分析，以鉴定可能参与疾病发展的 miRNA。发现了 50 个差异表达的 miRNA（DEM），其中 30 个在 VVD 患病鸟类中上调，20 个下调（|log2 Fold Change|≥1，-value<0.05）。DEM 与假定的靶基因匹配，发现了 864 个靶基因。这些靶基因的基因本体论（GO）分析表明，它们在“细胞质”术语中显著富集（-value<0.05），并且大多数靶基因在“细胞成分”中富集。京都基因与基因组百科全书（KEGG）分析表明，它们在 11 个信号通路中显著富集（-value<0.05），包括代谢途径、2-氧羧酸代谢、肌动蛋白细胞骨架调节、嘌呤代谢、内吞作用等。我们发现，它们在免疫相关途径中富集，其中 MAPK、Notch、JAK-Stat、Toll 样受体、p53 等单途径参与骨骼疾病的发生。我们之前的研究中获得的差异表达的 mRNAs 用于构建一个由 16 个 DEM 和 21 个差异表达的 mRNAs 组成的相互作用网络（|log2 Fold Change|≥1，-value<0.05）。我们发现，miR-12247-5p、miR-15c-5p、miR-15b-5p 和 位于网络相互作用的中心。这项研究为进一步研究 VVD 的发病机制和遗传机制提供了基础。
