假基因作为竞争性内源性 RNA：靶标预测与验证。

Pseudogenes as Competitive Endogenous RNAs: Target Prediction and Validation.

机构信息

Department of Molecular Oncology, H. Lee Moffitt Cancer Center and Research Institute, Tampa, FL, USA.

Department of Veterinary Sciences, University of Turin, Turin, Italy.

出版信息

Methods Mol Biol. 2021;2324:115-129. doi: 10.1007/978-1-0716-1503-4_8.

DOI:10.1007/978-1-0716-1503-4_8

PMID:34165712

Abstract

Pseudogenes may regulate expression of their parental genes as well as other protein-coding genes through various mechanisms. One such mechanism is the ability to act as competitive endogenous RNA (ceRNA) and participate in microRNA-mediated cross-regulation. Here, we outline how to predict the targets of pseudogene ceRNAs bioinformatically and how to validate them experimentally.

摘要

假基因可能通过多种机制调节其亲本基因以及其他蛋白质编码基因的表达。其中一种机制是作为竞争性内源性 RNA（ceRNA）的能力，并参与 microRNA 介导的交叉调节。在这里，我们概述了如何通过生物信息学预测假基因 ceRNA 的靶标，以及如何通过实验验证它们。