钙离子剥夺对分离的平滑肌细胞短期脱敏的影响，该平滑肌细胞对乙酰胆碱呈现全或无反应。

Effect of Ca2+ deprivation on short-term desensitization of isolated smooth muscle cells showing an all-or-none response to acetylcholine.

Mita M, Matsuo N, Uchida M K

Department of Molecular Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.

Gen Pharmacol. 1988;19(3):441-5. doi: 10.1016/0306-3623(88)90044-4.

Abstract

Short-term desensitization of the contractile response of isolated smooth muscle cells from guinea pig taenia caecum to acetylcholine was examined in the absence of external Ca2+. 2. Isolated smooth muscle cells contracted to 10(-7)-10(-6) M acetylcholine, showing an all-or-none response. 3. Desensitization was induced by incubating the cells with 10(-4) M acetylcholine for 10 min in Ca2+-free solution. 4. Desensitized cells showed an all-or-none response but required a higher concentration of acetylcholine for induction of contraction, indicating that the desensitization was due to a change in the threshold concentration. 5. Desensitization is suggested to be induced before massive influx of Ca2+ into the cells.

摘要

在无细胞外钙离子的情况下，研究了豚鼠盲肠带孤立平滑肌细胞对乙酰胆碱收缩反应的短期脱敏作用。2. 孤立平滑肌细胞对10（-7）-10（-6）M乙酰胆碱收缩，呈现全或无反应。3. 在无钙溶液中用10（-4）M乙酰胆碱孵育细胞10分钟可诱导脱敏。4. 脱敏细胞呈现全或无反应，但诱导收缩需要更高浓度的乙酰胆碱，表明脱敏是由于阈值浓度的改变。5. 提示脱敏作用在钙离子大量流入细胞之前就已诱导产生。