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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Mita M, Ono T, Hashimoto T, Uchida M K

Department of Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.

Gen Pharmacol. 1993 Sep;24(5):1085-90. doi: 10.1016/0306-3623(93)90353-y.

Single smooth muscle cells from the guinea-pig taenia caecum and the fundus of guinea-pig stomach were prepared by collagenase digestion under different, mild conditions. 2. Most of the cells either from taenia caecum or from the fundus of stomach responded repeatedly, showing an all-or-none response to acetylcholine (ACh). 3. The threshold concentrations of ACh were different for the cells of the two tissues. Although individual cells showed an all-or-none response to ACh, the average responses of all the cells were graded, like that of whole tissues. 4. Thus, isolated single smooth muscle cells from different tissues and under different conditions responded to ACh in an all-or-none manner such as the twitch observed in skeletal muscle. 5. These results suggest that the isolation of cells reveals the fundamental characteristics of smooth muscle cells as excitable.

在不同温和条件下，通过胶原酶消化制备豚鼠盲肠带和豚鼠胃底的单个平滑肌细胞。2. 来自盲肠带或胃底的大多数细胞都能反复做出反应，对乙酰胆碱（ACh）呈现全或无反应。3. 两种组织细胞对ACh的阈值浓度不同。尽管单个细胞对ACh呈现全或无反应，但所有细胞的平均反应是分级的，类似于整个组织的反应。4. 因此，在不同条件下从不同组织分离出的单个平滑肌细胞对ACh的反应呈全或无方式，类似于骨骼肌中观察到的抽搐。5. 这些结果表明，细胞的分离揭示了平滑肌细胞作为可兴奋细胞的基本特征。

Mita M, Ono T, Hashimoto T, Uchida M K

Department of Pharmacology, Meiji College of Pharmacy, Tokyo, Japan.

Gen Pharmacol. 1993 Sep;24(5):1085-90. doi: 10.1016/0306-3623(93)90353-y.

Single smooth muscle cells from the guinea-pig taenia caecum and the fundus of guinea-pig stomach were prepared by collagenase digestion under different, mild conditions. 2. Most of the cells either from taenia caecum or from the fundus of stomach responded repeatedly, showing an all-or-none response to acetylcholine (ACh). 3. The threshold concentrations of ACh were different for the cells of the two tissues. Although individual cells showed an all-or-none response to ACh, the average responses of all the cells were graded, like that of whole tissues. 4. Thus, isolated single smooth muscle cells from different tissues and under different conditions responded to ACh in an all-or-none manner such as the twitch observed in skeletal muscle. 5. These results suggest that the isolation of cells reveals the fundamental characteristics of smooth muscle cells as excitable.

在不同温和条件下，通过胶原酶消化制备豚鼠盲肠带和豚鼠胃底的单个平滑肌细胞。2. 来自盲肠带或胃底的大多数细胞都能反复做出反应，对乙酰胆碱（ACh）呈现全或无反应。3. 两种组织细胞对ACh的阈值浓度不同。尽管单个细胞对ACh呈现全或无反应，但所有细胞的平均反应是分级的，类似于整个组织的反应。4. 因此，在不同条件下从不同组织分离出的单个平滑肌细胞对ACh的反应呈全或无方式，类似于骨骼肌中观察到的抽搐。5. 这些结果表明，细胞的分离揭示了平滑肌细胞作为可兴奋细胞的基本特征。