苏丹分离的金黄色葡萄球菌 16s 核糖体 RNA 基因分子基础鉴定的初步研究。
First insights into molecular basis identification of 16 s ribosomal RNA gene of Staphylococcus aureus isolated from Sudan.
机构信息
Department of Medical Microbiology, Faculty of Medical Laboratory Sciences, University of Khartoum, Khartoum, Sudan.
Department of Medical Microbiology, Faculty of Medical Laboratory Sciences and the Director of Central Research Laboratory, University of Khartoum, Khartoum, Sudan.
出版信息
BMC Res Notes. 2021 Jun 25;14(1):240. doi: 10.1186/s13104-021-05569-w.
Abstract
OBJECTIVE
In this study, we analyzed the molecular evolution of Staphylococcus aureus isolates using 16S rRNA gene and phylogenetic analysis to detect the prevalence of S. aureus infections in Sudan.
RESULTS
Molecular detection of S. aureus has shown that 20 (43.47%) of patients were positive for S. aureus. The phylogenetic tree of 16S rRNA sequences was divided into three lineages of S. aureus isolates detected from wound infections in Sudan. Nucleotides base-pair substitution was appeared at position 249. This mutation do not linked with Macrolides, Lincosamides and Streptogramines b resistant phenotype. Further studies should investigate the effect of that mutation on resistance to other antibiotics.
摘要
目的
本研究通过 16S rRNA 基因和系统发育分析，分析金黄色葡萄球菌分离株的分子进化，以检测苏丹金黄色葡萄球菌感染的流行情况。
结果
金黄色葡萄球菌的分子检测显示，20 例（43.47%）患者金黄色葡萄球菌阳性。从苏丹伤口感染中检测到的 16S rRNA 序列系统发育树分为三个金黄色葡萄球菌分离株谱系。在位置 249 出现核苷酸碱基取代。这种突变与大环内酯类、林可酰胺类和糖肽类 b 耐药表型无关。进一步的研究应该调查这种突变对其他抗生素耐药性的影响。
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