Department of Microbiology, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, China.
Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.
Lett Appl Microbiol. 2021 Nov;73(5):646-651. doi: 10.1111/lam.13527. Epub 2021 Aug 30.
Emetic Bacillus cereus strains produce a potent cereulide cytotoxin, which can cause acute and fatal cases of food poisoning. We isolated 18 emetic B. cereus strains from a food poisoning event, and from clinical and non-random food surveillance in China and phenotypic characteristics of haemolysis, starch hydrolysis, salicin fermentation, gelatin liquefaction, cytotoxicity, and susceptibility to antibiotics were assessed. All isolates were positive for haemolysis and gelatin liquefaction, and negative for starch hydrolysis and salicin fermentation. Their haemolytic potentials were intermediate to Bacillus anthracis and B. cereus ATCC 14579 (a non-emetic strain). All isolates were cytotoxic to CHO, Hep-2, and Vero cells, and were sensitive to ampicillin. The homogeneous phenotypes of emetic isolates from China are similar to the corresponding traits of European and Japanese isolates that have been characterized, suggesting highly similar phenotypes of emetic B. cereus worldwide.
产吐毒素的蜡样芽胞杆菌菌株产生一种有效的呕吐毒素细胞毒素，可导致急性和致命的食物中毒。我们从食物中毒事件以及中国临床和非随机食品监测中分离出 18 株产吐毒素的蜡样芽胞杆菌菌株，并评估了其溶血、淀粉水解、水杨苷发酵、明胶液化、细胞毒性和抗生素敏感性等表型特征。所有分离株均对溶血和明胶液化呈阳性，对淀粉水解和水杨苷发酵呈阴性。它们的溶血潜能介于炭疽芽孢杆菌和蜡样芽胞杆菌 ATCC 14579（非产吐毒素菌株）之间。所有分离株对 CHO、Hep-2 和 Vero 细胞均具有细胞毒性，且对氨苄西林敏感。来自中国的产吐毒素分离株的同质表型与已鉴定的欧洲和日本分离株的相应特征相似，表明产吐毒素的蜡样芽胞杆菌在全球具有高度相似的表型。
