Wang Guowen, Sun Qianhui, Wang Huanling, Liu Hong
College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair/ Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China; Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, 430070, China.
College of Fisheries, Key Lab of Freshwater Animal Breeding, Ministry of Agriculture and Rural Affair/ Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, Huazhong Agricultural University, Wuhan, 430070, China; Engineering Research Center of Green Development for Conventional Aquatic Biological Industry in the Yangtze River Economic Belt, Ministry of Education, Wuhan, 430070, China.
Dev Comp Immunol. 2021 Nov;124:104185. doi: 10.1016/j.dci.2021.104185. Epub 2021 Jun 24.
Circular RNAs (circRNAs), a class of non-coding RNAs, play an important role in regulating various biological processes. In the present study, circRNAs from the Megalobrama amblycephala liver were identified at five different time points post Aeromonas hydrophila using RNA-seq technology. A total of 250 circRNAs were identified, of which 106 were differentially expressed (DE) in ten pairwise comparisons. GO and KEGG analyses showed that the parental genes of DE circRNAs were enriched in phagocytosis, complement and coagulation cascades, and Fc gamma R-mediated phagocytosis pathways. According to ceRNA hypothesis, the interaction network of circRNAs, miRNAs and mRNAs was constructed. Moreover, WGCNA was conducted, and five specific modules significantly related to bacterial infection were identified. All the above results reveal the important role of circRNAs in immune response, which enriches the information of circRNAs in teleost, and helps to understand the immune response mechanism of M. amblycephala to A. hydrophila.
环状RNA（circRNAs）是一类非编码RNA，在调控各种生物学过程中发挥着重要作用。在本研究中，利用RNA测序技术在嗜水气单胞菌感染后的五个不同时间点鉴定了团头鲂肝脏中的环状RNA。共鉴定出250个环状RNA，其中106个在十组两两比较中差异表达。基因本体（GO）和京都基因与基因组百科全书（KEGG）分析表明，差异表达环状RNA的亲本基因富集于吞噬作用、补体和凝血级联反应以及FcγR介导的吞噬作用途径。根据竞争性内源RNA（ceRNA）假说，构建了环状RNA、微小RNA（miRNA）和信使RNA（mRNA）的相互作用网络。此外，进行了加权基因共表达网络分析（WGCNA），并鉴定出五个与细菌感染显著相关的特定模块。上述所有结果揭示了环状RNA在免疫反应中的重要作用，丰富了硬骨鱼类环状RNA的信息，并有助于了解团头鲂对嗜水气单胞菌的免疫反应机制。
