College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Key Laboratory of Marine Biotechnology of Fujian Province, Institute of Oceanology, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, PR China; Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health, Fujian Agriculture and Forestry University, Fuzhou 350002, PR China.
Aquat Toxicol. 2021 Aug;237:105896. doi: 10.1016/j.aquatox.2021.105896. Epub 2021 Jun 14.
Curcumin is a polyphenol with antioxidant activity that has been used to protect the health of fish livers. Our previous studies about comparative transcriptome have shown that curcumin can enhance the Nrf2-Keap1 signaling pathway and induce downstream anti-stress genes to maintain cell viability. However, the possible role of miRNAs in the protective mechanism of curcumin is not understood. In this study, the tilapia hepatocyte HO stress model was used, and the miRNA expression profile for four groups (control group, curcumin group, HO group, and protection group) were established by high-throughput sequencing. In our results, 278-333 types of Oreochromis niloticus miRNAs, 309-543 types of conserved miRNAs, and 535-746 types of novel miRNAs were identified in different samples. Differentially expressed miRNAs were identified by comparing miRNA expression profiles among the four groups. The expression levels were confirmed by q-PCR. The target genes of these differentially expressed miRNAs were predicted, and their functional annotations were enriched by GO and KEGG analysis, which revealed that many target genes were involved in "response to stimulus" and "antioxidant activity" in each pair of groups. Several miRNAs related to oxidative stress showed differential expression. For example, in the HO group, the expression of miR-122 was decreased, and the expression of miR-21 and miR-489 increased significantly. In the curcumin group, the expression of miR-153b was decreased, and the expression of miR-200a and miR-29 was increased significantly. miR-153b, miR-200a, and miR-29 may be involved in the regulation of the Nrf2-Keap1 signaling pathway by curcumin. This work might provide insights into the molecular mechanisms of miRNA regulation of curcumin on the prevention and alleviation of liver diseases in fish.
姜黄素是一种具有抗氧化活性的多酚类物质，已被用于保护鱼类肝脏健康。我们之前的比较转录组研究表明，姜黄素可以增强 Nrf2-Keap1 信号通路，并诱导下游抗应激基因以维持细胞活力。然而，miRNA 在姜黄素保护机制中的可能作用尚不清楚。在本研究中，使用罗非鱼肝 HO 应激模型，通过高通量测序建立了对照组、姜黄素组、HO 组和保护组四个组的 miRNA 表达谱。在我们的结果中，在不同样品中鉴定出 278-333 种奥利亚罗非鱼 miRNA、309-543 种保守 miRNA 和 535-746 种新型 miRNA。通过比较四组间 miRNA 表达谱鉴定差异表达 miRNA，并通过 q-PCR 验证其表达水平。预测这些差异表达 miRNA 的靶基因，并通过 GO 和 KEGG 分析进行其功能注释，结果表明许多靶基因参与了每对组的“应激反应”和“抗氧化活性”。一些与氧化应激相关的 miRNA 表现出差异表达。例如，在 HO 组中，miR-122 的表达降低，而 miR-21 和 miR-489 的表达显著增加。在姜黄素组中，miR-153b 的表达降低，而 miR-200a 和 miR-29 的表达显著增加。miR-153b、miR-200a 和 miR-29 可能参与了姜黄素对 Nrf2-Keap1 信号通路的调节。这项工作可能为 miRNA 对鱼类肝脏疾病的预防和缓解的姜黄素调节的分子机制提供新的见解。
