Department of Biomedical Engineering, Columbia University, New York, New York, USA.
Department of Biomedical Engineering, Columbia University, New York, New York, USA; Department of Radiology, Columbia University, New York, New York, USA.
Ultrasound Med Biol. 2021 Sep;47(9):2759-2767. doi: 10.1016/j.ultrasmedbio.2021.04.030. Epub 2021 Jun 24.
Focused ultrasound (FUS) has been used to non-invasively elicit or inhibit motor neuronal activity in the mouse peripheral nervous system in vivo. However, less is known about whether FUS elicits immune system responses associated with peripheral sensory neuronal activity. In this study, we sought to determine that non-invasive ultrasound image-guided FUS can elicit the neurogenic axon reflex of peripheral nerves in the mouse sciatic nerve. The local vasodilation in the plantar view of the hind paw detected with a high-resolution laser Doppler imager indicated neurogenic flare responses after FUS stimulation. The effects of FUS were compared with control groups, where a distinct pattern of blood flow changes was observed only in FUS-elicited neurogenic flare responses. The findings indicate that image-guided FUS elicits local axon reflexes in vivo with a high degree of specificity and penetration depth.
聚焦超声（FUS）已被用于非侵入性地引出或抑制活体小鼠周围神经系统中的运动神经元活性。然而，对于 FUS 是否会引发与周围感觉神经元活性相关的免疫系统反应知之甚少。在这项研究中，我们试图确定非侵入性超声图像引导的 FUS 可以引发小鼠坐骨神经中的神经源性轴突反射。通过高分辨率激光多普勒成像仪在足底视图中检测到的局部血管扩张表明 FUS 刺激后会产生神经源性闪光反应。将 FUS 的作用与对照组进行了比较，只有在 FUS 引发的神经源性闪光反应中才观察到明显的血流变化模式。这些发现表明，图像引导的 FUS 以高度的特异性和穿透深度引发体内局部轴突反射。
