Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Taian, China.
Key Laboratory of Healthy Freshwater Aquaculture, Ministry of Agriculture and Rural Affairs; Key Laboratory of Fish Health and Nutrition of Zhejiang Province; Zhejiang Institute of Freshwater Fisheries, Huzhou, China.
Front Immunol. 2021 Jun 11;12:679767. doi: 10.3389/fimmu.2021.679767. eCollection 2021.
The hepatopancreas is an important digestive and immune organ in crustacean. There were low but stable numbers of microbes living in the hemolymph of crustacean, whereas the organs (including hepatopancreas) of crustacean were immersed in the hemolymph. It is very important to study the immune mechanism of the hepatopancreas against bacteria. In this study, a novel CTL (HepCL) with two CRDs, which was mainly expressed in the hepatopancreas, was identified in red swamp crayfish (). HepCL binds to bacteria and could enhance bacterial clearance . Compared with the C-terminal CRD of HepCL (HepCL-C), the N-terminal CRD (HepCL-N) showed weaker bacterial binding ability and stronger bacterial clearance activity . The expression of some antimicrobial proteins, such as FLP, ALF1 and ALF5, was downregulated under knockdown of HepCL or blocked with Anti-HepCL after challenge with in crayfish. These results demonstrated that HepCL might be involved in the antibacterial immune response by regulating the expression of antimicrobial proteins.
肝胰腺是甲壳动物重要的消化和免疫器官。甲壳动物的血淋巴中存在数量较少但稳定的微生物，而甲壳动物的器官（包括肝胰腺）则浸泡在血淋巴中。研究肝胰腺对细菌的免疫机制非常重要。在这项研究中，在红螯螯虾（）中鉴定出一种新型 CTL（HepCL），它具有两个 CRD，主要在肝胰腺中表达。HepCL 与细菌结合，并能增强细菌清除。与 HepCL 的 C 端 CRD（HepCL-C）相比，N 端 CRD（HepCL-N）显示出较弱的细菌结合能力和较强的细菌清除活性。在敲低 HepCL 或用 Anti-HepCL 阻断后用 处理螯虾，一些抗菌蛋白，如 FLP、ALF1 和 ALF5 的表达下调。这些结果表明，HepCL 可能通过调节抗菌蛋白的表达参与抗菌免疫反应。
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