Gao Xiaojian, Zhu Yujie, Qian Qieqi, Chen Anting, Qin Lijie, Tang Xinzhe, Jiang Qun, Zhang Xiaojun
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Animals (Basel). 2024 Oct 4;14(19):2864. doi: 10.3390/ani14192864.
is a significant cultivated species in China. However, decapod iridescent virus 1 (DIV1), as a newly discovered crustacean-lethal virus, has resulted in significant financial losses for the industry. In order to examine the immunological response of to DIV1, we conducted transcriptome analysis of the hepatopancreas from infected with DIV1 using RNA-seq. RNA sequencing analysis identified a combined total of 41,712 assembled unigenes, and 7014 genes that showed differential expression were identified in the group infected with DIV1, compared to the control group. Among these DEGs, 3952 were found to be up-regulated, while 3062 were down-regulated; many well-characterized DEGs were involved in innate immune defense, particularly involving the C-type lectin receptor signaling pathway, complement and coagulation cascades, phagosome, lysosome and PPAR signaling pathway. Moreover, the expression levels of well-known immune-related genes (, , , , , ) in the hepatopancreas and hemolymph were investigated by Quantitative real-time PCR (qRT-PCR), and the findings demonstrated a significant increase in gene expression in the hepatopancreas and hemolymph at various time points after infection. The results acquired in this study offered further comprehensive understanding of the immunological response of to DIV1 infection.
是中国一种重要的养殖品种。然而，十足目虹彩病毒1（DIV1）作为一种新发现的能导致甲壳类动物死亡的病毒，给该行业造成了重大经济损失。为了研究其对DIV1的免疫反应，我们使用RNA测序技术对感染DIV1的其肝胰腺进行了转录组分析。RNA测序分析共鉴定出41712个组装的单基因，与对照组相比，在感染DIV1的组中鉴定出7014个差异表达基因。在这些差异表达基因中，发现3952个上调，3062个下调；许多特征明确的差异表达基因参与先天免疫防御，特别是涉及C型凝集素受体信号通路、补体和凝血级联反应、吞噬体、溶酶体和PPAR信号通路。此外，通过定量实时PCR（qRT-PCR）研究了肝胰腺和血淋巴中知名免疫相关基因（、、、、、）的表达水平，结果表明感染后不同时间点肝胰腺和血淋巴中的基因表达显著增加。本研究获得的结果为进一步全面了解其对DIV1感染的免疫反应提供了依据。
