Soncrant T T, Holloway H W, Stipetic M, Rapoport S I
Laboratory of Neurosciences, National Institute on Aging, Bethesda, MD 20892.
J Cereb Blood Flow Metab. 1988 Oct;8(5):720-6. doi: 10.1038/jcbfm.1988.119.
The effects of immobilization and femoral artery and vein cannulation on resting rates of local cerebral glucose utilization (LCGU) were measured in 35 brain regions of awake rats by using the quantitative, autoradiographic [14C]2-deoxy-D-glucose [( 14C]DG) technique. Three groups of rats were cannulated on the previous day, and LCGU was measured under conditions of no restraint, 4 h of hindlimb restraint, or acute, four-limb immobilization. A fourth group represented the conventional preparation for [14C]DG experiments, with same-day cannulation followed immediately by 4 h of hindlimb restraint. Plasma catecholamines, corticosterone, and glucose concentrations were measured in all groups; all were elevated significantly above values in unrestrained animals only during four-limb immobilization. LCGU was unchanged by same-day surgery, hindlimb restraint, or both. During four-limb immobilization, LCGU was reduced by 25% in the dorsal hippocampus, and to a lesser extent in the anteroventral thalamic nucleus. It was increased only in the lateral habenula (42%). We conclude that two stressors of the experimental preparation (same-day surgery and hindlimb restraint) do not influence LCGU measurements by the [14C]DG method. More severe, acute stress selectively alters LCGU in a few rat brain regions.
采用定量放射自显影[14C]2-脱氧-D-葡萄糖（[14C]DG）技术，在清醒大鼠的35个脑区测量了制动以及股动脉和静脉插管对局部脑葡萄糖利用率（LCGU）静息率的影响。三组大鼠在前一天进行插管，分别在无束缚、后肢束缚4小时或急性四肢制动的条件下测量LCGU。第四组代表[14C]DG实验的传统准备方式，即当天插管后立即进行后肢束缚4小时。测量了所有组的血浆儿茶酚胺、皮质酮和葡萄糖浓度；仅在四肢制动期间，所有这些指标均显著高于未束缚动物的值。当天手术、后肢束缚或两者同时存在时，LCGU均未改变。在四肢制动期间，背侧海马体的LCGU降低了25%，前腹侧丘脑核的降低程度较小。仅外侧缰核的LCGU增加了（42%）。我们得出结论，实验准备的两个应激源（当天手术和后肢束缚）不会影响通过[14C]DG方法测量的LCGU。更严重的急性应激会选择性地改变大鼠一些脑区的LCGU。
