Ju Tingting, Willing Benjamin P
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Canada.
Bio Protoc. 2018 Mar 20;8(6):e2780. doi: 10.21769/BioProtoc.2780.
The colonization abundance of commensal in the gastrointestinal tract of healthy laboratory mice and rats ranges from 10 to 10 CFU/g feces. Although very well characterized, the family that belongs to has a very homogeneous 16S rRNA gene sequence, making the identification from 16S rRNA sequencing difficult. This protocol provides a procedure of isolating and identifying commensal strains from a healthy laboratory mouse or rat feces. The method can be applied to isolate commensal from other laboratory rodent strains.
健康实验小鼠和大鼠胃肠道中共生菌的定殖丰度范围为每克粪便10至10 CFU。尽管其特征非常明确，但它所属的家族具有非常一致的16S rRNA基因序列，这使得通过16S rRNA测序进行鉴定变得困难。本方案提供了一种从健康实验小鼠或大鼠粪便中分离和鉴定共生菌菌株的程序。该方法可应用于从其他实验啮齿动物菌株中分离共生菌。
