Department of Pharmacognosy Faculty of Pharmacy, Mansoura University, Mansoura, Egypt.
Division of Systematic Forest and Forest Products Sciences, Department of Agro-Environmental Sciences, Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Fukuoka, Japan.
Nat Prod Res. 2022 Oct;36(20):5134-5141. doi: 10.1080/14786419.2021.1922902. Epub 2021 Jun 28.
Two new compounds, 11-methoxy-11,12-dihydro phytuberin (2) and 9-methoxy-benzocyclononan-7-one (6), together with twenty-six known ones were isolated from (Solanaceae). Their planar structure was established by extensive spectroscopic analyses. The absolute configuration of compound 6 was determined by time dependent density functional theory calculations (TDDFT). The cytotoxic potential of the isolates was assessed in cultured skin cancer (G-361) and colon cancer (HCT-116 and CaCo-2) cell lines. Certain flavonoids showed the highest cytotoxic activity, with IC values ranging from 7.1 to 63.3µM; meanwhile 5-flurouracil showed IC values ranging from 62.4 to >100 µM. All compounds showed minimal toxicity towards normal cells from skin (NHDF-4) and colon (CCD-841), indicating their potential selectivity and safety as cytotoxic candidates.
从茄科植物中分离得到了两个新化合物，11-甲氧基-11,12-二氢千里光菲灵（2）和 9-甲氧基-苯并环壬酮（6），以及另外 26 个已知化合物。通过广泛的光谱分析确定了它们的平面结构。通过时间依赖密度泛函理论计算（TDDFT）确定了化合物 6 的绝对构型。对分离得到的化合物进行了体外细胞毒性实验，实验对象为皮肤癌细胞（G-361）、结肠癌细胞（HCT-116 和 CaCo-2）。某些类黄酮表现出最高的细胞毒性，IC 值范围为 7.1-63.3μM；而 5-氟尿嘧啶的 IC 值范围为 62.4->100μM。所有化合物对皮肤正常细胞（NHDF-4）和结肠正常细胞（CCD-841）的毒性最小，表明它们具有作为细胞毒性候选药物的潜在选择性和安全性。
