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载姜黄素壳聚糖纳米粒增强治疗藤黄微球菌感染的研究。

CD-g-CS nanoparticles for enhanced antibiotic treatment of Staphylococcus xylosus infection.

机构信息

College of Veterinary Medicine, Northeast Agricultural University, Harbin, Heilongjiang, 150030, China.

Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Harbin, Heilongjiang, 150030, China.

出版信息

Microb Biotechnol. 2022 Feb;15(2):535-547. doi: 10.1111/1751-7915.13870. Epub 2021 Jun 28.

DOI:10.1111/1751-7915.13870
PMID:34180582
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8867972/
Abstract

Staphylococcus xylosus (S. xylosus)-induced cow mastitis is an extremely serious clinical problem. However, antibiotic therapy does not successfully treat S. xylosus infection because these bacteria possess a strong biofilm formation ability, which significantly reduces the efficacy of antibiotic treatments. In this study, we developed ceftiofur-loaded chitosan grafted with β-cyclodextrins (CD-g-CS) nanoparticles (CT-NPs) using host-guest interaction. These positively charged nanoparticles improved bacterial internalization, thereby significantly improving the effectiveness of antibacterial treatments for planktonic S. xylosus. Moreover, CT-NPs effectively inhibited biofilm formation and eradicated mature biofilms. After mammary injection in a murine model of S. xylosus-induced mastitis, CT-NPs significantly reduced bacterial burden and alleviated inflammation, thereby achieving optimized therapeutic efficiency for S. xylosus infection. In conclusion, this treatment strategy could improve the efficiency of antibiotic therapeutics and shows great potential in the treatment of S. xylosus infections.

摘要

屎肠球菌（S. xylosus）诱导的奶牛乳腺炎是一个极其严重的临床问题。然而，抗生素治疗并不能成功治疗 S. xylosus 感染，因为这些细菌具有很强的生物膜形成能力，这大大降低了抗生素治疗的效果。在本研究中，我们利用主客体相互作用开发了载头孢噻呋的壳聚糖接枝β-环糊精（CD-g-CS）纳米颗粒（CT-NPs）。这些带正电荷的纳米颗粒促进了细菌的内化，从而显著提高了对浮游屎肠球菌的抗菌治疗效果。此外，CT-NPs 还能有效抑制生物膜的形成并清除成熟的生物膜。在屎肠球菌诱导的乳腺炎的小鼠模型中进行乳腺内注射后，CT-NPs 显著降低了细菌负荷并缓解了炎症，从而实现了对 S. xylosus 感染的优化治疗效果。综上所述，这种治疗策略可以提高抗生素治疗的效率，在治疗屎肠球菌感染方面具有很大的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/8867972/00d930dea472/MBT2-15-535-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/8867972/af0e832be876/MBT2-15-535-g007.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/8867972/922cf7f57506/MBT2-15-535-g006.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/8867972/c97f1b77c770/MBT2-15-535-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/8867972/103312aa7d07/MBT2-15-535-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/8867972/02c858eb729f/MBT2-15-535-g005.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/8867972/0d85868745c3/MBT2-15-535-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/fdef/8867972/00d930dea472/MBT2-15-535-g004.jpg

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