Bauer J, Hannig K
Max-Planck-Institut für Biochemie, Martinsried, F.R.G.
J Immunol Methods. 1988 Sep 13;112(2):213-8. doi: 10.1016/0022-1759(88)90360-2.
An elutriation system has been developed which fits into a small table-top centrifuge. The new CS1 rotor functioned as accurately as the JE-6 rotor, commercially available from Beckman Instruments. However, fewer cells were required for the separation experiments. Leukocytes obtained from 25 ml whole blood by Ficoll-Hypaque gradient centrifugation were sufficient for separating lymphocytes from monocytes and for purifying granulocytes. Furthermore, when lymphocytes were harvested from cell cultures 40 ml of a suspension of 1.2 x 10(6) cells/ml were enough to yield an enriched preparation of antibody producing cells.
已经开发出一种可适配小型台式离心机的淘析系统。新型CS1转子的运行精度与贝克曼仪器公司市售的JE - 6转子一样。然而，分离实验所需的细胞数量较少。通过Ficoll - Hypaque梯度离心从25毫升全血中获得的白细胞足以将淋巴细胞与单核细胞分离并纯化粒细胞。此外，当从细胞培养物中收获淋巴细胞时，40毫升每毫升含1.2×10⁶个细胞的悬浮液足以产生富含抗体产生细胞的制剂。
