Baker Sheridan J C, Van Der Kraak Glen
Department of Integrative Biology, University of Guelph, Ontario N1G 2W1, Canada.
Department of Integrative Biology, University of Guelph, Ontario N1G 2W1, Canada.
Gen Comp Endocrinol. 2021 Sep 15;311:113835. doi: 10.1016/j.ygcen.2021.113835. Epub 2021 Jun 26.
Prostaglandins (PGs) are a class of fatty-acid derived hormones that are essential in ovulation of teleosts, but their exact role remains unknown. One putative target of PGs in ovulation is regulation of the expression of members of the A Disintegrin and Metalloproteinase with Thrombospondin motifs (ADAMTS) family, which are implicated in follicular rupture. This study investigated the regulation of ADAMTS, other proteases, and their inhibitors in response to treatment with PGE or PGF. Four members of the ADAMTS family, ADAMTS1, ADAMTS5, ADAMTS9, and ADAMTS16 were shown to be expressed in the ovary of zebrafish, but only adamts1 was upregulated in full-grown follicles following treatment with PGE. Inhibitors of the PG receptors EP1 and EP2 had no effect on PGE-stimulated adamts1 expression, while treatment of full-grown follicles with both PGE and GW627368x, an inhibitor of EP4 function, prevented the PGE-induced increase in adamts1 expression. Treatment of full-grown follicles with the maturation-inducing hormone 17α,20β-dihydroxy-4-pregnen-3-one (17,20β-P) in vitro had no effect on the expression of adamts1 mRNA. These findings suggest that expression of ADAMTS1 in zebrafish ovarian follicles is regulated by the prostaglandin PGE via the EP4 series prostaglandin receptor.
前列腺素（PGs）是一类脂肪酸衍生的激素，在硬骨鱼排卵过程中至关重要，但其确切作用尚不清楚。PGs在排卵过程中的一个假定靶点是调节具有血小板反应蛋白基序的解整合素和金属蛋白酶（ADAMTS）家族成员的表达，这些成员与卵泡破裂有关。本研究调查了ADAMTS、其他蛋白酶及其抑制剂对PGE或PGF处理的反应调节情况。ADAMTS家族的四个成员，即ADAMTS1、ADAMTS5、ADAMTS9和ADAMTS16在斑马鱼卵巢中表达，但在用PGE处理后，只有adamts1在成熟卵泡中上调。PG受体EP1和EP2的抑制剂对PGE刺激的adamts1表达没有影响，而用PGE和EP4功能抑制剂GW627368x处理成熟卵泡，则可阻止PGE诱导的adamts1表达增加。体外使用成熟诱导激素17α,20β-二羟基-4-孕烯-3-酮（17,20β-P）处理成熟卵泡对adamts1 mRNA的表达没有影响。这些发现表明，斑马鱼卵巢卵泡中ADAMTS1的表达受前列腺素PGE通过EP4系列前列腺素受体调控。
