Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Division of Polar Life Science, Korea Polar Research Institute, Incheon 21990, South Korea.
Comp Biochem Physiol C Toxicol Pharmacol. 2021 Oct;248:109120. doi: 10.1016/j.cbpc.2021.109120. Epub 2021 Jun 25.
iTRAQ proteomic profiling was conducted to examine the proteomic responses of the Antarctic copepod Tigriopus kingsejongensis under ultraviolet B (UVB) exposure. Of the 5507 proteins identified, 3479 proteins were annotated and classified into 25 groups using clusters of orthologous genes analysis. After exposing the T. kingsejongensis to 12 kJ/m UVB radiation, 77 biological processes were modulated over different time periods (0, 6, 12, 24, and 48 h) compared with the control. A Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis showed that UVB exposure in T. kingsejongensis downregulated ribosome and glyoxylate and dicarboxylate metabolism at all time points. Furthermore, antioxidant and chaperone proteins were highly downregulated in response to UVB exposure, causing protein damage and activating apoptotic processes in the 48 h UVB exposure group. These proteomic changes show the mechanisms that underlie the detrimental effects of UVB on the cellular defense systems of the Antarctic copepod T. kingsejongensis.
采用 iTRAQ 蛋白质组学分析方法研究了南极桡足类动物秀丽白虾在中波紫外线（UVB）暴露下的蛋白质组应答反应。在鉴定的 5507 种蛋白质中，通过同源基因聚类分析将 3479 种蛋白质注释并分类为 25 组。将秀丽白虾暴露于 12 kJ/m2 的 UVB 辐射下，与对照组相比，在不同时间点（0、6、12、24 和 48 h）有 77 个生物学过程发生了变化。京都基因与基因组百科全书通路富集分析表明，UVB 暴露使秀丽白虾的核糖体和乙醛酸和二羧酸代谢在所有时间点下调。此外，抗氧化和伴侣蛋白在对 UVB 的应答中高度下调，导致蛋白质损伤并在 48 h UVB 暴露组中激活凋亡过程。这些蛋白质组学变化显示了 UVB 对南极桡足类动物秀丽白虾细胞防御系统产生有害影响的潜在机制。
