白细胞介素-4 拮抗外周血单个核细胞对激素敏感型前列腺癌细胞的细胞毒性作用。
IL-4 Counteracts the Cytotoxic Effects of Peripheral Blood Mononuclear Cells on Hormone-sensitive Prostate Cancer Cells.
机构信息
Department of Urology, Technische Universität Dresden, Dresden, Germany.
UroFors Consortium (Natural Scientists in Urological Research) of the German Society of Urology, Düsseldorf, Germany.
出版信息
In Vivo. 2021 Jul-Aug;35(4):1973-1977. doi: 10.21873/invivo.12465.
BACKGROUND/AIM: Proinflammatory cytokines play an essential role in the development and progression of prostate cancer (PCa). Especially interleukine (IL-)6 is involved in the development of aggressive PCa. Peripheral blood mononuclear cells (PBMC) have been reported to interact with cancer cells and subsequently lead to increased production of pro-inflammatory cytokines. However, the role of anti-nflammatory cytokines, such as IL-4 is still largely unexplored in prostate cancer. In the present study, we investigated the effects of IL-4 on PBMC co-cultured with PCa cells.
MATERIALS AND METHODS
PBMC were co-culured with the PCa cell lines LNCaP and LNCaP-IL6+. To avoid cell-cell contact, cancer and immune cells were separated using cell culture inserts with a 0.4 μm pore size membrane. Cell growth was assessed using the [3-(4, 5-dimethylthiazol-2-yl)-2, 5 diphenyl tetrazolium bromide] (MTT) assay. Cytokine levels were measured using a BD™Cytometric Bead Array.
RESULTS
Cell viability of LNCaP cells decreased massively when cells were co-cultured with PBMC. Pre-incubation with IL-4 could partly rescue the observed effect of cell viability of LNCaP cells co-cultured with PBMC. In contrast, cell viability of the LNCaP-IL6+ cell line was not affected when co-cultured with PBMC.
CONCLUSION
IL-4 counteracts the cytotoxic effects of PBMC on hormone-sensitive LNCaP cells and is involved in the immune escape and development of aggressive phenotypes of PCa.
背景/目的：促炎细胞因子在前列腺癌（PCa）的发展和进展中起着至关重要的作用。特别是白细胞介素（IL-）6 参与了侵袭性 PCa 的发展。据报道，外周血单核细胞（PBMC）与癌细胞相互作用，随后导致促炎细胞因子的产生增加。然而，抗炎细胞因子（如 IL-4）在前列腺癌中的作用仍在很大程度上未被探索。在本研究中，我们研究了 IL-4 对与 PCa 细胞共培养的 PBMC 的影响。
材料和方法
将 PBMC 与 PCa 细胞系 LNCaP 和 LNCaP-IL6+共培养。为避免细胞-细胞接触，使用具有 0.4μm 孔径膜的细胞培养插入物将癌细胞和免疫细胞分离。使用[3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四唑溴盐]（MTT）测定法评估细胞生长。使用 BD™Cytometric Bead Array 测量细胞因子水平。
结果
当细胞与 PBMC 共培养时，LNCaP 细胞的细胞活力大大降低。用 IL-4 预处理可以部分挽救与 PBMC 共培养的 LNCaP 细胞观察到的细胞活力降低的影响。相比之下，当与 PBMC 共培养时，LNCaP-IL6+细胞系的细胞活力不受影响。
结论
IL-4 抵消了 PBMC 对激素敏感的 LNCaP 细胞的细胞毒性作用，并参与了 PCa 的免疫逃逸和侵袭性表型的发展。
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