抗凝剂在直接膜喂养检测中对恶性疟原虫传播的影响。
The effect of anticoagulants in blood collection tubes on Plasmodium falciparum transmission in direct membrane feeding assays.
机构信息
Radboud University Medical Center , Radboud Institute for Health Sciences , Department of Medical Microbiology, 268, PO Box 9101, 6500 HB Nijmegen, The Netherlands.
Department of Immunology and Infection, London School of Hygiene and Tropical Medicine, Keppel St, London WC1E 7HT, UK.
出版信息
Trans R Soc Trop Med Hyg. 2022 Feb 1;116(2):187-189. doi: 10.1093/trstmh/trab095.
BACKGROUND
Direct membrane feeding assays assess the transmission potential of malaria-infected individuals using whole blood collected in anticoagulant vacutainers.
METHODS
The potential inhibitory effect of four commonly used anticoagulants on gametocyte infectivity to mosquitoes was assessed in standard membrane feeding assays with cultured Plasmodium falciparum.
RESULTS
Infection burden in mosquitoes was significantly reduced when blood was collected in sodium citrate and EDTA. Transmission was highest when blood was collected in lithium heparin and sodium heparin, although a concentration-dependent inhibition of mosquito infection was also observed.
CONCLUSIONS
Although anticoagulants can reduce transmission efficiency, lithium heparin and sodium heparin are the best anticoagulants for evaluating malaria transmission.
背景
直接膜喂养检测使用抗凝采血管采集的全血来评估疟疾感染者的传播潜力。
方法
在使用培养的恶性疟原虫进行的标准膜喂养检测中，评估了四种常用抗凝剂对疟原虫感染蚊子的潜在抑制作用。
结果
当血液用柠檬酸钠和 EDTA 采集时，蚊子的感染负担明显降低。当血液用肝素锂和肝素钠采集时，传播率最高，尽管也观察到了蚊子感染的浓度依赖性抑制。
结论
尽管抗凝剂可以降低传播效率，但肝素锂和肝素钠是评估疟疾传播的最佳抗凝剂。
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