Rey-Martínez Natalia, Merdan Gökçe, Guisasola Albert, Baeza Juan Antonio
GENOCOV. Departament d'Enginyeria Química, Biològica i Ambiental, Universitat Autònoma de Barcelona, Spain.
GENOCOV. Departament d'Enginyeria Química, Biològica i Ambiental, Universitat Autònoma de Barcelona, Spain; Department of Environmental Engineering, Namık Kemal University, Turkey.
Chemosphere. 2021 Nov;283:131173. doi: 10.1016/j.chemosphere.2021.131173. Epub 2021 Jun 15.
Enhanced biological phosphorus removal (EBPR) is an efficient and sustainable technology to remove phosphorus from wastewater. A widely known cause of EBPR deterioration in wastewater treatment plants (WWTPs) is the presence of nitrate/nitrite or oxygen in the anaerobic reactor. Moreover, most existing studies on the effect of either permanent aerobic conditions or inhibition of EBPR by nitrate or free nitrous acid (FNA) have been conducted with a "Candidatus Accumulibacter" or Tetrasphaera-enriched sludge, which are the two major reported groups of polyphosphate accumulating organisms (PAO) with key roles in full-scale EBPR WWTPs. This work reports the denitrification capabilities of a bio-P microbial community developed using glutamate as the sole source of carbon and nitrogen. This bio-P sludge exhibited a high denitrifying PAO (DPAO) activity, in fact, 56% of the phosphorus was uptaken under anoxic conditions. Furthermore, this mixed culture was able to use nitrite and nitrate as electron acceptor for P-uptake, being 1.8 μg HNO-N·L the maximum FNA concentration at which P-uptake can occur. Net P-removal was observed under permanent aerobic conditions. However, this microbial culture was more sensitive to FNA and permanent aerobic conditions compared to "Ca. Accumulibacter"-enriched sludge.
强化生物除磷（EBPR）是一种从废水中去除磷的高效且可持续的技术。污水处理厂（WWTPs）中EBPR性能恶化的一个广为人知的原因是厌氧反应器中存在硝酸盐/亚硝酸盐或氧气。此外，大多数关于永久好氧条件或硝酸盐或游离亚硝酸（FNA）对EBPR抑制作用的现有研究都是使用“Candidatus Accumulibacter”或富含Tetrasphaera的污泥进行的，这是在全规模EBPR污水处理厂中发挥关键作用的两组主要报道的聚磷积累生物体（PAO）。这项工作报告了以谷氨酸作为唯一碳源和氮源培养的生物除磷微生物群落的反硝化能力。这种生物除磷污泥表现出较高的反硝化聚磷菌（DPAO）活性，事实上，在缺氧条件下56%的磷被吸收。此外，这种混合培养物能够利用亚硝酸盐和硝酸盐作为磷吸收的电子受体，磷吸收能够发生的最大FNA浓度为1.8 μg HNO-N·L。在永久好氧条件下观察到了净磷去除。然而，与富含“Ca. Accumulibacter”的污泥相比，这种微生物培养物对FNA和永久好氧条件更敏感。
