Felipo V, Miñana M D, Azorín I, Grisolía S
Instituto de Investigaciones Citológicas de la Caja de Ahorros de Valencia, Spain.
J Neurochem. 1988 Oct;51(4):1041-5. doi: 10.1111/j.1471-4159.1988.tb03065.x.
The effect of oral administration of ammonium acetate for 2, 15, 30, and 100 days on protein synthesis in rat brain was investigated. Although protein synthesis changes were modest, i.e., maximal increase of 24%, there was induction of synthesis and accumulation of a protein with an Mr of 55,000. We show, on the basis of its position on two-dimensional electrophoresis and its immunological reactivity, that this protein is tubulin. Its content increased by 33% as determined by isolation of tubulin after 15 days of oral administration of ammonium and to 49% after 100 days as determined by quantitative immunoblotting.
研究了口服醋酸铵2天、15天、30天和100天对大鼠脑内蛋白质合成的影响。尽管蛋白质合成变化不大，即最大增加24%，但诱导合成并积累了一种分子量为55,000的蛋白质。基于其在二维电泳上的位置及其免疫反应性，我们表明这种蛋白质是微管蛋白。口服醋酸铵15天后，通过微管蛋白分离测定其含量增加了33%，100天后通过定量免疫印迹测定增加到49%。
